中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 2018-2021.doi: 10.3969/j.issn.1673-8225.2011.11.029

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

氯化胆碱与制动性骨骼肌萎缩肌肉生成抑制素mRNA的表达

秦开元1，寇建民2，木拉提别克1，刁和信1   

  1. 1伊犁师范学院体育学院，新疆维吾尔自治区伊宁市  835000
    2河南大学体育学院，河南省开封市 475001
  • 收稿日期:2010-09-05 修回日期:2010-10-30 出版日期:2011-03-12 发布日期:2011-03-12
  • 作者简介:秦开元★，男，1972 年生，江苏人，2006年东北师范大学毕业，硕士，讲师，主要从事体育社会学和运动营养学方面的研究。 qky20095@163.com

Effects of choline chloride on myostatin mRNA expression in rats with immobilization-induced skeletal muscle atrophy

Qin Kai-yuan1, Kou Jian-min2, Mu Latibieke1, Diao He-xin1   

  1. 1Department of Physical Education, Yili Normal University, Yining  833500, Xinjiang Uigur Autonomous Region, China
    2College of Physical Education, Henan University, Kaifeng   475001, Henan Province, China
  • Received:2010-09-05 Revised:2010-10-30 Online:2011-03-12 Published:2011-03-12
  • About author:Qin Kai-yuan★, Master, Lecturer, Department of Physical Education, Yili Normal University, Yining 833500, Xinjiang Uigur Autonomous Region, China qky20095@163.com

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摘要：

背景：研究发现类胆碱物质可增加乙酰胆碱的弥散及终板电流的幅度，对神经肌肉接点功能退化有一定的对抗作用。
目的：观察氯化胆碱对制动性骨骼肌萎缩的防治作用及对骨骼肌萎缩大鼠肌肉生成抑制素mRNA表达的影响。
方法：将30只雄性SD大鼠随机分为对照组、模型组和治疗组，每组10只。采用可塑性石膏固定模型组和治疗组大鼠右后肢制备肌萎缩模型。治疗组每日灌胃氯化胆碱(150 mg/kg)，对照组和模型组灌胃等体积蒸馏水。4周后解剖右后肢腓肠肌，检测腓肠肌收缩张力、肌湿质量、蛋白质水平及肌肉生成抑制素mRNA的表达。
结果与结论：与对照组比较，模型组大鼠腓肠肌的收缩张力、肌湿质量、蛋白质水平均显著降低(P < 0.05或P < 0.01)，肌肉生成抑制素mRNA表达显著增高(P < 0.01)。与模型组比较，治疗组大鼠腓肠肌的收缩张力、肌湿质量、蛋白质水平均显著升高(P < 0.05)，肌肉生成抑制素mRNA表达显著降低(P < 0.05)。说明氯化胆碱能够显著提高制动性萎缩骨骼肌的收缩张力、肌湿质量、蛋白质水平，减少肌肉生成抑制素mRNA的表达，从而有效抑制骨骼肌制动性萎缩的发生。

关键词: 制动, 骨骼肌, 萎缩, 氯化胆碱, 肌肉生成抑制素

Abstract:

BACKGROUND: Previous studies demonstrated that choline-based material can not only increase dispersion of acetylcholine and amplitude of endplate current, but also play an antagonistic action on function reduction of neuromuscular junction.
OBJECTIVE: To observe the prevention effects of choline chloride on immobilization-induced skeletal muscle atrophy and myostatin mRNA expression.
METHODS: Thirty male SD rats were divided into control group, model group and treatment group, with 10 rats in each group. The right hind limbs of the rats were fixed with compliant plaster to prepare atrophy models. The rats in the treatment group received choline chloride (150 mg/kg). The same volume of distilled water was intragastric administrated into rats of the control and model groups. After 4 weeks, calf muscle in right hind limb were dissected and measured for the contractility, wet weight, protein content, and myostatin mRNA expression.
RESULTS AND CONCLUSION: Compared with the control group, the contractility, wet weight, and protein levels in the calf muscle of the model group were dramatically decreased (P < 0.05 or P < 0.01), but the myostatin mRNA expression was obviously increased (P < 0.01). Compared with the model group, the contractility, wet weight, and protein levels in the calf muscle of the treatment group were dramatically increased (P < 0.05), and the myostatin mRNA expression was obviously decreased (P < 0.01). The findings demonstrated that, choline chloride can improve contractility, wet weight, and protein levels in the calf muscles, and decrease myostatin mRNA expression, accordingly, choline chloride has preventive and protective effects on development of disuse atrophy of skeletal muscles.

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