中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 2013-2017.doi: 10.3969/j.issn.1673-8225.2011.11.028

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

兔深静脉血栓形成与血浆单核细胞趋化蛋白4及白细胞介素8的水平

付召军，任  为   

  1. 重庆医科大学附属第一医院血管外科，重庆市  400016
  • 收稿日期:2010-11-25 修回日期:2011-01-11 出版日期:2011-03-12 发布日期:2011-03-12
  • 通讯作者: 任为，副教授，重庆医科大学附属第一医院血管外科，重庆市 400016 www.renwei9771@yahoo.com
  • 作者简介:付召军★，男，1984年生，四川省眉山市人，汉族，重庆医科大学在读硕士，主要从事血栓的防治与复发机制研究。 xiangminhuacq@163.com

Plasma monocyte chemoattractant protein 4 and interleukin-8 levels in rabbit deep venous thrombosis 

Fu Zhao-jun, Ren Wei   

  1. Department of Vascular Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing  400016, China
  • Received:2010-11-25 Revised:2011-01-11 Online:2011-03-12 Published:2011-03-12
  • Contact: Ren Wei, Associate professor, Department of Vascular Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China www.renwei9771@ yahoo.com
  • About author:Fu Zhao-jun★, Stufying for master’s degree, Department of Vascular Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China xiangminhuacq@163.com

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457

被引次数

1

摘要：

背景：关节置换后深静脉血栓形成的预后在个体间存在很大的差异。有研究表明，巨噬细胞和内皮细胞活性的增高对于血栓溶解管腔再通有促进作用。
目的：分析兔深静脉血栓形成后不同时段血浆单核细胞趋化蛋白4及白细胞介素8质量浓度对于反映血栓病情有无特异性。
方法：新西兰大白兔78只，随机分为血栓组、假手术组及正常对照组，血栓组造模后14 d根据血栓再通情况分为再通组及未通组。分别于血栓后8 h，3 d，7 d，14 d抽取耳缘静脉血行单核细胞趋化蛋白4、白细胞介素8质量浓度测定和血常规检测。
结果与结论：血栓后第7天血浆单核细胞趋化蛋白4质量浓度再通组较未通组有显著升高(P < 0.01)，白细胞介素8质量浓度再通组较未通组降低(P < 0.05)。其变化与单核细胞、中性粒细胞之间无相关性(P > 0.05)。结果表明血栓形成后血浆高质量浓度的单核细胞趋化蛋白4的病例血栓再通情况较好。单核细胞趋化蛋白4的质量浓度通过反映巨噬细胞和内皮细胞的活性，在一定程度上反映了深静脉血栓形成的预后。

关键词: 静脉血栓, 再通, 单核细胞趋化蛋白4, 白细胞介素8, 相关性

Abstract:

BACKGROUND: There are grate individual difference in prognosis of deep venous thrombosis (DVT). Studies have showed that macrophage and endothelial cell play a great part in recanalization.
OBJECTIVE: To study the rabbit plasma monocyte chemoattractant protein 4 (MCP-4) and interleukin-8 (IL-8) levels in different time with DVT, and to observe whether there is specificity in reflection the thrombus.
METHODS: Totally 78 New Zealand rabbits were divided into thrombosis group, sham-surgery group, and normal group. According to the thrombus at 14 days after operation, the thrombosis group was divided into recanalization group and non-recanalization group. The plasma MCP-4 and IL-8 levels were measured and blood routine was performed at 8 hours, 3, 7, and 14 days after operation.
RESULTS AND CONCLUSION: Plasma MCP-4 levels were obviously increased in the recanalization group at 7 days after operation (P < 0.01), but the IL-8 levels were decreased compared with the non-recanalization group (P < 0.05). The variations were not correlated with monocytes or neutrophils (P > 0.05). It is suggested that there is better recanalization results in thrombus that with higher plasma MCP-4. MCP-4 levels can forecast thrombosis prognosis via reflecting activity of macrophage and endothelial cells.

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