中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 2006-2008.doi: 10.3969/j.issn.1673-8225.2011.11.026

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

光动力学照射小鼠牙周组织的病理变化

杜  波1，蒋  琳2，刘明方3，李苹苹1，林居红1   

  1. 重庆医科大学附属口腔医院，1牙体牙髓科，2预防科，重庆市 400015，3重庆医科大学病原微生物教研室，重庆市 400015
  • 收稿日期:2010-08-28 修回日期:2010-09-26 出版日期:2011-03-12 发布日期:2011-03-12
  • 通讯作者: 林居红，教授，主任医师，硕士生导师，主要从事龋病的研究。 juhonglin@sina.com
  • 作者简介:杜波★，男，1982年生，山东省临沂市人，汉族，重庆医科大学在读硕士，主要从事龋病的研究。 03-0958@163.com
  • 基金资助:

    重庆市卫生局基金资助项目(2008-2-243)，光动力学疗法对人工菌斑生物膜影响的实验研究。

Pathological changes of mouse periodontal tissues following photodynamic therapy

Du Bo1, Jiang Lin2, Liu Ming-fang3, Li Ping-ping1, Lin Ju-hong1   

  1. 1Department of Endodontics, 2Department of Prevention, Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing   400015, China;  3Department of Pathogenic Microorgamnism, Chongqing Medical University, Chongqing   400015, China
  • Received:2010-08-28 Revised:2010-09-26 Online:2011-03-12 Published:2011-03-12
  • Contact: Lin Ju-hong, Professor, Chief physician, Master’s supervisor Department of Endodontics, Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400015, China juhonglin@sina.com
  • About author:Du Bo★, Studying for Master‘s degree, Department of Endodontics, Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400015, China 03-0958@163.com
  • Supported by:

    the Foundation of Chongqing Health Bureau, No. 2008-2-243*

PDF

398

被引次数

3

摘要：

背景：光化学疗法对口腔组织可能具有伤害，在临床应用甲苯胺蓝O介导的光动力学疗法预防龋病前，需进行生物安全性评估。
目的：观察甲苯胺蓝介导的光动力学疗法产生的光敏作用对小鼠牙周组织健康的影响。
方法：将54只小鼠随机分为9组，分别用0，50，100 mg/L 甲苯胺蓝O联合二极管光照0，15，25 min。作用后72 h，切取牙周组织切片观察。
结果与结论：经光照治疗后小鼠的牙龈、牙槽骨和牙齿没有发现有坏死、溃疡、增生等异常改变。提示甲苯胺蓝介导的光动力学疗法一个安全的预防龋病的方法，对牙齿临近的牙周组织无破坏性影响。

关键词: 甲苯胺蓝, 二极管激光, 光动力学疗法, 小鼠, 牙周组织, 病理

Abstract:

BACKGROUND: Photodynamic therapy (PDT) may have bad effects on oral cavity tissue, so the biological safety evaluation is necessary before Toluidine blue O (TBO)-mediated PDT of dentine caries in clinic.
OBJECTIVE: To observe the effects of photosensitization of the TBO-mediated PDT to periodontal tissues in mice.
METHODS: Fifty-four mice were randomly divided into nine groups, and were respectively treated with 0 mg/L, 50 mg/L, 100 mg/L TBO with 0, 15, 25min LED illumination. Periodontal tissue slices were taken to observation after treatment of 72 hours.
RESULTS AND CONCLUSION: There were no abnormal changes like necrosis, ulcer or hyperplasia happens in the mice gingival, alveolar bone or teeth after light treatment. This indicated that TBO-mediated PDT was safe to the prevention of dental caries, without damaging effect to periodontal tissues adjacent to the teeth.

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