中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 2002-2005.doi: 10.3969/j.issn.1673-8225.2011.11.025

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

RPMI-1640和DMEM培养基中肝癌细胞系BEL-7402与HepG-2的生长状态比较

赵  翔，张  沙，肖军军，林  明   

  1. 北京大学医学部细胞生物系，北京市  100191
  • 收稿日期:2010-10-29 修回日期:2011-01-21 出版日期:2011-03-12 发布日期:2011-03-12
  • 通讯作者: 林明，副主任技师，北京大学医学部细胞生物系，北京市 100191 linminga@bjmu.edu.cn 并列通讯作者：肖军军，副教授，北京大学医学部细胞生物系，北京市 100191 xiaojunjun@ bjmu.edu.cn
  • 作者简介:赵翔，女，1961年生，北京市人，汉族，主管技师，主要从事抗癌药物的作用机制与分子和细胞生物学方面的研究。 xiangzh@bjmu. edu.cn
  • 基金资助:

    北京大学985细胞生物学重点学科建设项目。

Growth status of BEL-7402 and HepG-2 cells in RPMI-1640 versus DMEM media

Zhao Xiang, Zhang Sha, Xiao Jun-jun, Lin Ming   

  1. Department of Cell Biology, Peking University Health Science Center, Beijing  100191, China
  • Received:2010-10-29 Revised:2011-01-21 Online:2011-03-12 Published:2011-03-12
  • Contact: Lin Ming, Associate chief technician, Department of Cell Biology, Peking University Health Science Center, Beijing 100191, China linminga@bjmu. edu. cn Correspondence to: Xiao Jun-jun, Associate professor, Department of Cell Biology, Peking University Health Science Center, Beijing 100191, China xiaojunjun@bjmu. edu.cn
  • About author:Zhao Xiang, Technician-in-charge, Department of Cell Biology, Peking University Health Science Center, Beijing 100191, China xiangzh@bjmu. edu.cn
  • Supported by:

    the 985 Key Subject of Cell Biology in Peking University*

PDF

1284

被引次数

8

摘要：

背景：DMEM和RPMI-1640是两种最常用的商品化培养基，二者对肝癌细胞生长的影响尚未见直接的对比研究。
目的：比较两种常用培养基对人肝癌BEL-7402与HepG-2细胞系体外生长和增殖效果的影响，从中选择更适合的培养基。
方法：分别应用高糖DMEM与RPMI-1640完全培养液培养BEL-7402和HepG-2细胞，于培养的0，24，48，72，96，  120 h用酸性磷酸酶检测法测定细胞生长和增殖速率，并于倒置显微镜下观察细胞的形态。
结果与结论：结果发现BEL-7402和HepG-2细胞在RPMI-1640培养基中的生长增殖速率均明显高于DMEM培养基 (P < 0.01)。镜下观察证实细胞在RPMI-1640培养基中的黏附和伸展状态更好。因此，建议首选RPMI-1640培养基进行肿瘤细胞体外培养。

关键词: 细胞培养基, 完全培养液, 肝癌细胞系, 生长状态, 酸性磷酸酶检测法, 细胞活率

Abstract:

BACKGROUND: The most popular commercialized media are DMEM and RPMI-1640. Yet direct comparative study of the two popular media on the influence of liver cancer cell growth has not been reported.
OBJECTIVE: Compare the effect of two popular culture media on growth and proliferation of human hepatocarcinoma BEL-7402 and HepG2 cells, in order to find a more suitable medium.
METHODS: BEL-7402 and HepG2 cells were cultured in complete growth media prepared with either the high glucose-DMEM or the RPMI-1640 medium. Growth and proliferation rates were detected at timed intervals by the acid phosphatase assay. Cellular morphology was observed under an inverse light microscope.
RESULTS AND CONCLUSION: Compared with the DMEM medium, BEL-7402 and HepG2 cells cultured in the RPMI-1640 medium showed a significantly higher growth and proliferation rates (P < 0.01). The cells also revealed better adhesive and stretching status in the RPMI-1640 medium, as confirmed under a microscope. The RPMI-1640 medium is the first choice for tumor cell cultures.

中图分类号: