中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 1981-1985.doi: 10.3969/j.issn.1673-8225.2011.11.020

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

烫伤创面愈合后的瘢痕形成与神经支配

郭鹏飞1，张  芮2，冯永强1，朱守荣1，王一兵1   

  1. 1山东大学附属省立医院烧伤整形外科，山东省济南市 250021
    2山东医学高等专科学校病理与病理生理教研室，山东省济南市250002
  • 收稿日期:2010-10-21 修回日期:2011-01-15 出版日期:2011-03-12 发布日期:2011-03-12
  • 通讯作者: 王一兵，博士，主任医师、教授、硕士生导师，山东大学附属省立医院烧伤整形外科，山东省济南市 250021 wyb0616@ yahoo.com.cn
  • 作者简介:郭鹏飞★，男，1984年生，河南省三门峡市人，山东大学在读硕士。 gpf19840329@yahoo.com.cn
  • 基金资助:

    课题受山东省自然科学基金(Y2002C29)资助。

Effect of innervation on scar formation after burn wound healing

Guo Peng-fei1, Zhang Rui2, Feng Yong-qiang1, Zhu Shou-rong1, Wang Yi-bing1   

  1. 1Department of Burn and Plastic Surgery, Provincial Hospital Affiliated to Shandong University, Jinan  250021, Shandong Province, China; 2Department of Pathology and Pathophysiology, Shandong Medical College, Jinan  250021, Shandong Province, China
  • Received:2010-10-21 Revised:2011-01-15 Online:2011-03-12 Published:2011-03-12
  • Contact: Wang Yi-bing, Doctor, Professor, Chief physician, Master’s supervisor, Department of Burn and Plastic Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China wyb0616@yahoo. com.cn
  • About author:Guo peng-fei★, Studying for master’s degree, Department of Burn and Plastic Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China
  • Supported by:

    the Natural Science Foundation of Shandong Province, No. Y2002C29*

PDF

522

被引次数

11

摘要：

背景：既往研究表明神经因素在创面愈合中具有重要调控作用，但有关神经调节与创面愈合后瘢痕形成之间的关系鲜有报道。
目的：观察烧伤创面愈合过程中神经支配与创面愈合质量之间的关系。
方法：将30只大鼠右侧T9~L1阶段脊神经根切断，制作失神经支配皮肤模型；然后在大鼠背部右侧失神经支配皮肤区域制作直径4 cm的深Ⅱ度烫伤创面，设为模型组，左侧对称正常皮肤制作同样的创面，设为对照组，伤后连续观察创面变化，于7，14，21 d采用免疫组织化学法观察Ⅰ和Ⅲ胶原分泌情况，并计算Ⅰ/Ⅲ型胶原比例的变化，探讨创面愈合速度及愈合质量。
结果与结论：模型组Ⅰ，Ⅲ型胶原分泌和Ⅰ/Ⅲ型胶原比值于伤后各时间点均显著低于对照组(P < 0.05)。模型组Ⅰ型胶原分泌与伤后7，14，21 d逐渐增加(P < 0.05)，Ⅲ型胶原于伤后21 d时分泌明显增高(P < 0.05)，Ⅰ/Ⅲ型胶原比值与伤后21 d时明显降低(P < 0.05)。结果证实，创面早期神经支配可以促进创面愈合，创面重塑期减轻神经支配可能会改善创面重塑质量。

关键词: 神经支配, 创面愈合, 胶原蛋白, 瘢痕, 烫伤, 组织构建

Abstract:

BACKGROUND: Previous experiments show that neural factors play important regulating roles in wound healing, however, the relationship between neural regulation and scar formation after wound healing is known little.
OBJECTIVE: To observe the relationship between innervations and wound healing in the process of scalded wound healing.
METHODS: Right T9-L1 stage spinal nerve roots of 30 rats were cut off and prepared for denervation skin models; and then a diameter of 4 cm and a depth ofⅡdegree scalded wound was make on the right denervated skin area of rats (model group), the same wounds were made on the left normal skin as control group. The changes of wounds were continuously observed. The secretion of type Ⅰ, Ⅲ collagens were observed by immunohistochemical method, and type Ⅰ/Ⅲ collagen ratios were calculated, the speed and quality of wound healing was explored.
RESULTS AND CONCLUSION: Compared with the control group, the type I/III collagen ratio was lower in the model group (P < 0.05). Secretion of type Ⅰ collagen gradually increased in the model group at 7, 14 and 21 days after injury (P < 0.05), and type III collagen was significantly increased at 21 days after injury (P < 0.05). Type Ⅰ / Ⅲcollagen ratio was significantly decreased at 21 days after injury (P < 0.05). It is revealed that early innervation of the wound may promote wound healing, and alleviating innervation on wound remodeling stage may improve the quality of wound remodeling.

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