中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 1965-1968.doi: 10.3969/j.issn.1673-8225.2011.11.016

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

长波紫外线照射皮肤成纤维细胞表皮生长因子受体的表达

刘  垠1，刘  流1，王继华2，鲁开化3，伍尚敏4，杨瑞年1，龙庭凤1，袁瑞宏1   

  1. 1昆明医学院第一附属医院整形外科，云南省昆明市650032
    2昆明医学院第二附属医院整形外科，云南省昆明市650101
    3解放军第四军医大学第一附属医院整形外科，陕西省西安市 710032
    4昆明市第一人民医院整形外科，云南省昆明市650011
  • 收稿日期:2011-01-04 修回日期:2011-02-02 出版日期:2011-03-12 发布日期:2011-03-12
  • 通讯作者: 刘流，博士，教授，博士生导师，昆明医学院第一附属医院、云南省口腔颌面外科研究中心，昆明医学院第一附属医院整形外科，云南省昆明市 650032
  • 作者简介:刘垠☆，男，1978 年生，云南省楚雄禄丰县人，汉族，昆明医学院在读博士，医师，主要从事颜面部美容和皮肤老化研究。 liuyinsky@163. com
  • 基金资助:

    云南省自然科学基金面上项目(2004C0047M)。

Influence of ultraviolet light A on epidermal growth factor receptor expression in fibroblasts in vitro

Liu Yin1, Liu Liu1, Wang Ji-hua2, Lu Kai-hua3, Wu Shang-min4, Yang Rui-nian1, Long Ting-feng1, Yuan Rui-hong1   

  1. 1Department of Plastic Surgery, the First Affiliated Hospital of Kunming Medical University, Kunming  650032, Yunnan Province, China
    2Department of Plastic Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming  650101, Yunnan Province, China
    3Department of Plastic Surgery, the First Affiliated Hospital of Fourth Military Medical University, Xi’an  710032, Shaanxi Province, China
    4Department of Plastic Surgery, the First People’s Hospital of Kunming, Kunming  650011, Yunnan Province, China
  • Received:2011-01-04 Revised:2011-02-02 Online:2011-03-12 Published:2011-03-12
  • Contact: Liu Liu, Doctor, Professor, Doctoral supervisor, Department of Plastic Surgery, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
  • About author:Liu Yin☆, Studying for doctorate, Physician, Department of Plastic Surgery, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China liuyinsky@163.com
  • Supported by:

    the Natural Sciences Foundation General Projects of Yunnan Province, No. 2004C0047M*

PDF

525

被引次数

10

摘要：

背景：长波紫外线与人体皮肤老化关系密切，主要作用于表皮和真皮全层。
目的：观察长波紫外线照射对体外培养的皮肤成纤维细胞的表皮生长因子受体表达的影响。
方法：体外培养人成纤维细胞，将细胞分为对照组、长波紫外线照射5，10，20 J/cm2组。照射24 h后，分别用实时PCR和Western blot检测表皮生长因子受体mRNA和蛋白的表达情况。
结果与结论：体外培养的皮肤成纤维细胞随着长波紫外线照射剂量的增加，表皮生长因子受体的mRNA与蛋白水平均显著增加，提示长波紫外线可以诱导成纤维细胞里的表皮生长因子的表达。

关键词: 长波紫外线, 光老化, 表皮生长因子受体, 成纤维细胞, 皮肤

Abstract:

BACKGROUND: Ultraviolet A has a close relationship with skin photoaging, which impact on epidermis and dermis.
OBJECTIVE: To explore the influence of ultraviolet A on the epidermal growth factor receptor expression of human skin fibroblasts in vitro.
METHODS: Adult’s fibroblasts were divided into the control and ultraviolet A irradiation (5, 10, 20 J/cm2) groups. The epidermal growth factor receptor mRNA and protein were detected by real-time PCR and Western blot.
RESULTS AND CONCLUSION: The epidermal growth factor receptor mRNA and protein levels had upgraded as the dose of ultraviolet A irradiation increased. It suggests that ultraviolet A can lead to epidermal growth factor receptor expression in fibroblasts.

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