中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 1961-1964.doi: 10.3969/j.issn.1673-8225.2011.11.015

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

椎间注射转化生长因子β1对兔退变腰椎间盘蛋白多糖表达的影响

吴  彬1，张  辉2，王海滨1，贾存岭1，赵益峰1   

  1. 1济宁医学院附属医院骨外科，山东省济宁市  272000
    2 泰山医学院附属医院骨外科，山东省泰安市  271000
  • 收稿日期:2010-09-28 修回日期:2010-11-02 出版日期:2011-03-12 发布日期:2011-03-12
  • 作者简介:吴彬★，男，1980年生，山东省滕州市人，汉族，2006年泰山医学院毕业，硕士，主治医师，主要从事脊柱外科方面的研究。 wb0902@163. com

Effects of intervertebral injection of transforming growth factor beta 1 on proteoglycan expression in rabbit degenerated lumbar discs

Wu Bin1, Zhang Hui2, Wang Hai-bin1, Jia Cun-ling1, Zhao Yi-feng1   

  1. 1Department of Orthopedics, Affiliated Hospital of Jining Medical College, Jining  272000, Shandong Province, China
    2 Department of Orthopedics, Affiliated Hospital of Taishan Medical College, Taian  271000, Shandong Province, China
  • Received:2010-09-28 Revised:2010-11-02 Online:2011-03-12 Published:2011-03-12
  • About author:Wu Bin★, Master, Attending physician, Department of Orthopedics, Affiliated Hospital of Jining Medical College, Jining 272000, Shandong Province, China wb0902@163.com

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摘要：

背景：体外研究证实转化生长因子β1可以促进蛋白多糖合成，延缓椎间盘退变，但体内实验鲜见报道。
目的：观察局部应用转化生长因子β1对兔退变腰椎间盘髓核蛋白多糖表达的影响。
方法：取30只新西兰大白兔建立兔腰椎间盘退变模型，造模12周，经X射线证实退变后，随机选择6只模型兔及6只未造模正常兔，处死取材。分别向剩余24只模型兔L4~5椎间隙注射转化生子因子β1和生理盐水。末次给药2，4周取材，间苯三酚法测定兔椎间盘髓核内蛋白多糖的水平。
结果与结论：造模12周，兔退变椎间盘髓核中蛋白多糖水平明显降低(P < 0.01)。经局部注射转化生子因子β1后，兔退变椎间盘髓核中蛋白多糖表达明显增多(P < 0.01)。说明在兔椎间注射转化生子因子β1 可以促进髓核蛋白多糖的合成，延缓椎间盘退变。

关键词: 椎间盘退变, 蛋白多糖, 转化生长因子&beta, 1, 兔, 动物模型

Abstract:

BACKGROUND: In vitro experiments have confirmed that transforming growth factor-β1 (TGF-β1) can promote proteoglycan synthesis and delay intervertebral disc degeneration, but its in vivo role remains unclear. 
OBJECTIVE: To study the effect of local utilization of TGF-β1 on proteoglycan expression in rabbit degenerated lumbar discs.
METHODS: Thirty-six New Zealand white rabbits were divided into two groups randomly, with 6 in control group and 30 in model group. The lumbar structure was destroyed partly in the model group. After 12 weeks, the intervertebral disc degeneration was testified by X-ray in model group. Six rabbits chosen randomly from the model group and all rabbits in the control group were killed and the organizations of the intervertebral discs of L4-5 were harvested. Meanwhile, the rest rabbits in model group were divided into two groups randomly, which was injected with TGF-β1 saline. The rabbits were killed by 2 and 4 weeks after injection. The contents of proteoglycan in nucleus pulposus were tested by phloroglucinol method. 
RESULTS AND CONCLUSION: The content of proteoglycan in the nucleus pulposus was obviously decreased at 12 weeks after model preparation (P < 0.01). After TGF-β1 injection, the content of proteoglycan was notably increased (P < 0.01). It is suggested that intervertebral injection of TGF-β1 to rabbit can delay intervertebral disc degeneration and enhance the synthesis of proteoglycan in nucleus pulposus.

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