中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 1953-1956.doi: 10.3969/j.issn.1673-8225.2011.11.013

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

糖尿病性骨质疏松模型雌激素、一氧化氮及转化生长因子β1的变化

刘中浩，高红伟，邢德国，赵锡武，王若义，宫明智   

  1. 山东大学第二医院骨外一科，山东省济南市250033
  • 收稿日期:2010-09-14 修回日期:2010-11-02 出版日期:2011-03-12 发布日期:2011-03-12
  • 通讯作者: 宫明智，博士，主任医师，山东大学第二医院骨外一科，山东省济南市 250033 gongmzh242@sina.com
  • 作者简介:刘中浩★，男，1969年生，山东省定陶县人，汉族，2003年西安交通大学毕业，硕士，主治医师，主要从事脊柱、创伤以及糖尿病骨质疏松的基础与临床研究。 liuxia990620@163.com
  • 基金资助:

    山东省科技发展计划项目(2008GG30002073)。

Changes of estrogen, nitric oxide and transforming growth factor beta 1 in rats with diabetic osteoporosis

Liu Zhong-hao, Gao Hong-wei, Xing De-guo, Zhao Xi-wu, Wang Ruo-yi, Gong Ming-zhi   

  1. First Department of Orthopaedics Surgery, Second Hospital of Shandong University, Jinan  250033, Shandong Province, China
  • Received:2010-09-14 Revised:2010-11-02 Online:2011-03-12 Published:2011-03-12
  • Contact: Gong Ming-zhi, Doctor, Chief physician, First Department of Orthopaedics Surgery, Second Hospital of Shandong University, Jinan 250033, Shandong Province, China gongmzh242@sina.com
  • About author:Liu Zhong-hao★, Master, Attending physician, First Department of Orthopaedics Surgery, Second Hospital of Shandong University, Jinan 250033, Shandong Province, China liuxia990620@163.com
  • Supported by:

    the Plan of Science and Technology Development of Shandong Province, No. 2008GG 30002073*

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摘要：

背景：糖尿病和卵巢去势是骨质疏松发生的两大主要原因，均会出现一氧化氮和转化生长因子β1的变化。
目的：探讨雌激素、一氧化氮、转化生长因子β1在糖尿病性骨质疏松模型中的变化及意义。
方法：采用链脲佐菌素诱导制备糖尿病大鼠模型，于造模后4，8，12，16周，进行骨密度检测，并应用放免法检测血清雌激素水平，硝酸还原酶法检测血清一氧化氮水平，ELISA法检测血清转化生长因子β1水平，免疫组织化学法检测骨中转化生长因子β1水平。
结果与结论：随着链脲佐菌素诱导时间的延长，糖尿病大鼠股骨骨密度逐渐降低(P < 0.05或P < 0.01)；血清一氧化氮水平逐渐降低(P < 0.05或P < 0.01)；血清转化生长因子β1水平逐渐升高(P < 0.01)；血清雌激素水平轻度降低，与同时间点正常大鼠比较差异无显著性意义(P > 0.05)。免疫组织化学结果显示转化生长因子β1主要表达于成骨细胞的胞浆中，其阳性表达随链脲佐菌素诱导时间的延长逐渐下降(P < 0.01)。说明糖尿病大鼠血清一氧化氮水平和骨组织中转化生长因子β1水平的变化导致了骨吸收增加，骨形成减少，使骨密度降低，参与了糖尿病性骨质疏松的发生。

关键词: 糖尿病性骨质疏松, 大鼠, 雌激素, 一氧化氮, 转化生长因子&beta, 1

Abstract:

BACKGROUND: Diabetes mellitus and ovariectomy are two main reasons for osteoporosis, both of which can lead to variations in nitric oxide (NO) and transforming growth factor β1 (TGF-β1).
OBJECTIVE: To study the changes of estrogen, NO, and TGF-β1 in the rats with diabetic osteoporosis.
METHODS: Rats with diabetic osteoporosis were induced by streptozotocin (STZ). The bone mineral density (BMD) was measured at 4, 8, 12 and 16 weeks after model preparation; the serum estrogen levels was measured by radioimmunoassay method, serum NO levels ere detected by nitrate reduction method, serum TGF-β1 levels determined by ELISA method, and the content of TGF-β1 in the bone tissues was measured by the immunohistochemical method.
RESULTS AND CONCLUSION: With induction time prolonged, BMD of femur was gradually decreased (P < 0.05 or P < 0.01), serum NO level decreased (P < 0.05 or P < 0.01), serum TGF-β1 level increased (P < 0.01). The estrogen level in the model group was slightly decreased, but the difference had no significance (P > 0.05). Immunohistochemical results showed that TGF-β1 was mainly expressed in the osteoblast cytoplasm, and the number of positive cells was gradually decreased with induction time prolonged (P < 0.01). The changes of serum NO and TGF-β1 in bone tissues result in lower bone mineral density because of increasing bone absorption and decreasing bone formation. Nitric oxide and TGF-β1 play an important role in diabetic osteoporosis.

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