中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 1949-1952.doi: 10.3969/j.issn.1673-8225.2011.11.012

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

亚急性创伤性深静脉血栓形成模型大鼠基质金属蛋白酶3的表达

章玉冰1，胡继红2，李  文3，姚黎清4，赵学凌4，陈  淼4，宋  恩4，李云健4，王  辉4，郑燕科4   

  1. 1昆明医学院2008级博士研究生，云南省昆明市  650032；昆明医学院第一附属医院，2放射科， 4骨科，云南省昆明市  650032；3云南省第一人民医院心内科，云南省昆明市  650032
  • 收稿日期:2010-12-15 修回日期:2011-01-19 出版日期:2011-03-12 发布日期:2011-03-12
  • 通讯作者: 李文，副主任医师，云南省人民医院心内科，云南省昆明市 650032
  • 作者简介:章玉冰☆，男，1970年生，安徽省宣城市人，汉族，昆明医学院在读博士，主要从事创伤骨科方面的研究。 zyb701017@ sina.com 并列第一作者：胡继红，男，1967年生，昆明医学院在读博士，副教授，主要从事影像及放射介入工作。
  • 基金资助:

    昆明医学院研究生创新基金项目资助(2009D02)。

Expression of matrix metalloproteinase-3 in a rat model of subtraumatic deep vein thrombosis

Zhang Yu-bing1, Hu Ji-hong2, Li Wen3, Yao Li-qing4, Zhao Xue-ling4, Chen Miao4, Song En4, Li Yun-jian4, Wang Hui4, Zheng Yan-ke4   

  1. 1Doctoral Student of Kunming Medical University, Kunming  650032, Yunnan Province, China; 2Department of Radiology, 4Department of Orthopaedics, First Affiliated Hospital of Kunming Medical University, Kunming   650032, Yunnan Province, China; 3Department of Cardiology, Yunnan First People’s Hospital, Kunming  650032, Yunnan Province, China
  • Received:2010-12-15 Revised:2011-01-19 Online:2011-03-12 Published:2011-03-12
  • Contact: Li Wen, Associate chief physician, Department of Cardiology, Yunnan First People’s Hospital, Kunming 650032, Yunnan Province, China
  • About author:Zhang Yu-bing☆, Studying for doctorate, Doctoral Student of Kunming Medical University, Kunming 650032, Yunnan Province, China zyb701017@sina. com Hu Ji-hong, Studying for doctorate, Associate professor, Department of Radiology, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China Zhang Yu-bing and Hu Ji-hong contributed equally to this paper.
  • Supported by:

    the Innovation Foundation for Post-graduates of Kunming Medical University, No. 2009D02*

PDF

499

被引次数

1

摘要：

背景：创伤性深静脉血栓发生率和死亡率呈逐年递增趋势，但其发生发展机制尚未清楚。因此有必要采用先进的基因芯片技术对其进行研究，探索其发生发展的内在规律。
目的：观察创伤性深静脉血栓形成过程中基质金属蛋白酶3的表达变化规律，探讨其在创伤性深静脉血栓形成中的作用机制。
方法：从110只SD大鼠中随机取10只大鼠作为正常组，其余100只大鼠作为实验组，采用定量击打双侧大腿+髋人字石膏固定方式，建立大鼠亚急性创伤性深静脉血栓动物模型。在伤后即刻、24，72，120和168 h时相点切取股静脉血管组织，随后抽取总RNA，采用Affymetrix 230 2.0芯片对股静脉血管组织进行基因表达检测。
结果与结论：在亚急性创伤性深静脉血栓形成演化过程中，基质金属蛋白酶3在实验组的表达随伤后时间的延长而增加，说明其参与血栓形成过程，可能是影响深静脉血栓生物学状态的主要因素之一。

关键词: 深静脉血栓形成, 大鼠, 亚急性创伤, 基因芯片, 基质金属蛋白酶3

Abstract:

BACKGROUND: Incidence and mortality of traumatic deep vein thrombosis (TDVT) gradually increased in recent years, which generation mechanism remains unclear. Accordingly, it is necessary to explore inherent rules using gene chip technology.
OBJECTIVE: To explore the changes and rules of matrix metalloproteinases in the TDVT. 
METHODS: Ten SD rats selected from 110 served as normal controls, other 100 rats were prepared subtraumatic TDVT models by beating on bilateral posterior limbs combined with hip spica cast fixation. The femoral vein was incised at immediately, 24, 72, 120 and 168 hours after operation. Total RNA was extracted from femoral vein, and the gene expression was detected by Affymetrix 230 2.0 genechips.
RESULTS AND CONCLUSION: Expression of matrix metalloproteinase-3 was increased with prolongation of damage duration in the experimental group, suggesting matrix metalloproteinase-3 can be one of the most major factors to influence the biological states of TDVT.

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