中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 1945-1948.doi: 10.3969/j.issn.1673-8225.2011.11.011

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

小胶质细胞介导帕金森病模型小鼠的炎症损伤

陆明佳1，朱  沂2，孙  娟2，杨雪荣2   

  1. 1新疆医科大学，新疆维吾尔自治区乌鲁木齐市 830001
    2新疆维吾尔自治区人民医院神经内科，新疆维吾尔自治区乌鲁木齐市 830001
  • 收稿日期:2010-10-19 修回日期:2011-01-10 出版日期:2011-03-12 发布日期:2011-03-12
  • 通讯作者: 朱沂，硕士，主任医师，博士生导师，新疆维吾尔自治区人民医院神经内科，新疆维吾尔自治区乌鲁木齐市 830001 zhuyixinjiang@ hotmail.com
  • 作者简介:陆明佳★，女，1984年生，江苏省人，新疆医科大学在读硕士，汉族，医师，主要从事神经保护研究。 circulation_0511@163.com
  • 基金资助:

    课题受新疆维吾尔自治区自然科学基金(2010211A56)资助，课题名称：新疆雪莲注射液的神经保护作用及其机制研究。

Microglia mediates inflammation injury in mouse models of Parkinson’s disease

Lu Ming-jia1, Zhu Yi2, Sun Juan2, Yang Xue-rong2   

  1. 1Xinjiang Medical University, Urumqi  830001, Xinjiang Uygur Autonomous Region, China
    2Department of Neurology, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi  830001, Xinjiang Uygur Autonomous Region, China
  • Received:2010-10-19 Revised:2011-01-10 Online:2011-03-12 Published:2011-03-12
  • Contact: Zhu Yi, Master, Chief physician, Doctoral supervisor, Department of Neurology, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, Xinjiang Uygur Autonomous Region, China zhuyixinjiang@ hotmail.com
  • About author:Lu Ming-jia★, Studying for master’s degree, Physician, Xinjiang Medical University, Urumqi 830001, Xinjiang Uygur Autonomous Region, China circulation_0511@ 163.com
  • Supported by:

    the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 2010211A56*

PDF

605

被引次数

22

摘要：

背景：研究表明帕金森病的黑质纹状体存在小胶质细胞的激活，但其释放炎症因子在帕金森病发病过程中的作用尚不明确。
目的：观察小胶质细胞激活所释放的两种炎症因子整合素α及肿瘤坏死因子α在小鼠黑质部位的蛋白表达及酪氨酸羟化酶的蛋白表达。
方法：将C57BL/6小鼠经腹腔注射百草枯(10 mg/kg)，设为帕金森病模型组，并设置对照组，观察两组小鼠行为活动。用高效液相法测定小鼠黑质纹状体多巴胺的含量。采用免疫组织化学法检测黑质部位酪氨酸羟化酶、整合素α及肿瘤坏死因子α蛋白表达。
结果与结论：百草枯可造成帕金森病模型组小鼠运动减少，并伴有运动迟缓、震颤、探嗅、竖毛及尾巴硬类似于帕金森病样的行为表现，并且脑内黑质纹状体多巴胺含量降低(P < 0.05)，酪氨酸羟化酶蛋白表达降低(P < 0.05)，整合素α和肿瘤坏死因子α蛋白表达增加(P < 0.05)，肿瘤坏死因子α在模型组小鼠黑质纹状体有阳性表达，且主要分布在小胶质细胞上。结果表明小胶质细胞介导的炎症损伤参与了帕金森多巴胺能神经元的损害过程。

关键词: 帕金森病, 百草枯, 小胶质细胞, 酪氨酸羟化酶, 肿瘤坏死因子&alpha, 整合素&alpha

Abstract:

BACKGROUND: Studies have demonstrated that microglia is activated in substantia nigra and striatum of Parkinson’s disease. However, the effect of released inflammatory factor on the invasion of Parkinson’s disease remains poorly understood.
OBJECTIVE: To investigate the expression of tyrosine hydroxylase (TH), integrin α and tumor necrosis factor α (TNF-α) mediated by microglia in mouse models of Parkinson’s disease.
METHODS: C57BL/6 mice were randomly divided into model group and control group. The model of Parkinson’s disease was established by peritoneal injection of paraquat to mice (10 mg/kg). Adult spontaneous motor activity was observed respectively. The level of dopamine in the substantia nigra and striatum was observed by high-performance liquid chromatography (HPLC). The expression of TH, integrin α and TNF-α in the substantia nigra pars compacta was measured by immunohistochemical staining.
RESULTS AND CONCLUSION: Compared with the control group, paraquat resulted in mouse showed a marked bypoactive behavior, such as bradykinesia, tremor, sniff and the level of dopamine in the substantia nigra and striatum was notably decreased (P < 0.05), TH declined (P < 0.05), but integrin α and TNF-α expressions increased (P < 0.05). TNF-α was positive expressed in the model group, which mainly distributed on the microglia. The inflammation injury which was mediated by microglia maybe involved in dopamine neuron damage of Parkinson’s disease.

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