中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 1909-1912.doi: 10.3969/j.issn.1673-8225.2011.11.003

• 骨组织构建 bone tissue construction • 上一篇    下一篇

离体骨的置入式微波消融

柯  晋，尹庆水，张  余，张  涛，徐  亮   

  1. 解放军广州军区广州总医院骨科，广东省广州市  510010
  • 收稿日期:2010-10-21 修回日期:2011-01-24 出版日期:2011-03-12 发布日期:2011-03-12
  • 通讯作者: 尹庆水，硕士，主任医师，博士生导师。解放军广州军区广州总医院骨科，广东省广州市 510010 yqi@medmail. com.cn
  • 作者简介:柯晋★，男，1983年生，江西省九江市人，汉族，南方医科大学在读硕士，主要从事骨肿瘤疾病方向的研究。 kejin332500@ yahoo.com.cn
  • 基金资助:

    广东省医学科研基金(B2009211)。

Implanted microwave ablation of bone in vitro

Ke Jin, Yin Qing-shui, Zhang Yu, Zhang Tao, Xu Liang   

  1. Department of Orthopaedics, Guangzhou General Hospital of Guangzhou Area Command of Chinese PLA, Guangzhou  510010, Guangdong Province, China
  • Received:2010-10-21 Revised:2011-01-24 Online:2011-03-12 Published:2011-03-12
  • Contact: Yin Qing-shui, Master, Chief physician, Doctoral supervisor, Department of Orthopaedics, Guangzhou General Hospital of Guangzhou Area Command of Chinese PLA, Guangzhou 510010, Guangdong Province, China yqi@medmail.com.cn
  • About author:Ke Jin★, Studying for master’s degree, Department of Orthopaedics, Guangzhou General Hospital of Guangzhou Area Command of Chinese PLA, Guangzhou 510010, Guangdong Province, China kejin332500@yahoo.com.cn
  • Supported by:

    the Medical Scientific Research Foundation of Guangdong Province, No. B2009211*

PDF

482

被引次数

3

摘要：

背景：微波高温可直接灭活骨肿瘤细胞，并且可维持骨的支架作用。
目的：探讨置入式微波对离体状态下正常骨组织的消融作用。
方法：采用微波消融治疗仪对新鲜离体猪骨干骺端进行消融，功率分别为60，70，80，90，100 W，消融时间300 s，微波频率2 450 MHz。刻度尺测量凝固区最长直径及最短直径；取凝固区标本，光镜及电镜观察其组织形态及超微结构。
结果与结论：置入式微波在不同功率下消融形态基本恒定，纵面上看类似一个椭球形，横面上看类似一个圆形，微波消融范围随着功率的增加明显增大，光镜下未见变化，电镜下可见经微波消融后的骨组织骨细胞核消失、空泡化，呈现完全坏死。说明置入式微波对离体状态下正常骨组织有消融作用。

关键词: 微波, 消融, 电镜, 光镜, 坏死, 骨组织

Abstract:

BACKGROUND: Microwave high temperature can deactivate bone tumor cells directly and maintain bone supporting effect. 
OBJECTIVE: To investigate the ablation effects of implanted microwave on bone in vitro.
METHODS: The rigid implanted microwave antenna with internally water-cooled were directly implanted into fresh procine bone in vitro. Microwave power was 60, 70, 80, 90 and 100 W, ablation duration was 300 seconds, and frequency was 2450MHz. The maximum and minimum diameter of congeal area was measured. The histomorphology and ultrastructure of congeal area specimen were observed by light microscope and electron microscope.
RESULTS AND CONCLUSION: The bone showed a reproducible lesion. The shapes of ablation lesions were elliptical in the vertical plane while rotund in the horizontal plane, maximum longitudinal and minimum longitudinal of ablation lesions were analyzed and Pathological and ultrastructural changes of the bone were observed, respectively. The ablation size enlarged with the increase of irradiation powers in differential degree. No changes can be observed in pathological. The nucleus disappeared and showed irreversible damage under electron microscope. It demonstrates that implanted microwave has ablation effects on bone in vitro.

中图分类号: