中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (3): 465-467.doi: 10.3969/j.issn.1673-8225.2011.03.021

• 生物材料基础实验 basic experiments of biomaterials • 上一篇    下一篇

低相对分子质量透明质酸脂质体的制备及透皮性能

张文强1,2，黄岳山1   

  1. 1华南理工大学生物科学与工程学院，广东省广州市 510061
    2深圳清华大学研究院，广东省深圳市 518057
  • 收稿日期:2010-08-07 修回日期:2010-12-07 出版日期:2011-01-15 发布日期:2011-01-15
  • 通讯作者: 黄岳山，博士，副教授，华南理工大学生物科学与工程学院，广东省广州市 510061
  • 作者简介:张文强★，男，1985年生，陕西省大荔县人，汉族，硕士，主要从事生物医用材料研究。 yywenzi23@163.com

Preparation of low-molecular-weight hyaluronan liposomes and their percutaneous penetration

Zhang Wen-qiang1,2, Huang Yue-shan1   

  1. 1Biological Science and Engineering College, South China University of Technology, Guangzhou  510006, Guangdong Province, China
    2Research Institute of Tsinghua University in Shenzhen, Shenzhen  518057, Guangdong Province, China
  • Received:2010-08-07 Revised:2010-12-07 Online:2011-01-15 Published:2011-01-15
  • Contact: Huang Yue-shan, Doctor, Associate professor, Biological Science and Engineering College, South China University of Technology, Guangzhou 510006, Guangdong Province, China
  • About author:Zhang Wen-qiang★, Master, Biological Science and Engineering College, South China University of Technology, Guangzhou 510006, Guangdong Province, China; Research Institute of Tsinghua University in Shenzhen, Shenzhen 518057, Guangdong Province, China yywenzi23@163.com

PDF

2167

被引次数

15

摘要：

背景：透明质酸是一种用途广泛的高分子材料，在实际应用中，由于其相对分子质量较大，透皮吸收效果不理想，利用脂质体作为其载体可以改善其传输效果，具有较好的应用前景。
目的：观察包裹低相对分子质量透明质酸脂质体的透皮性能。
方法：采用薄膜法制备低相对分子质量透明质酸脂质体，设计正交实验，采用蛇蜕研究其透皮吸收性能。
结果与结论: 温度35 ℃、胆固醇与卵磷脂的比例为0.15∶1，透明质酸与卵磷脂的比例为0.03∶1、水合介质PBS的pH值为7.5，可制备包封率较高的低相对分子质量透明质酸脂质体；脂质体包裹低相对分子质量透明质酸，能有效得提高透明质酸透过蛇蜕的能力，达到了提高透明质酸透皮吸收率的作用。

关键词: 低相对分子质量, 透明质酸, 脂质体, 透皮吸收, 包封率

Abstract:

BACKGROUND: Hyaluronic acid is a versatile polymer material, in practical applications, due to its large molecular weight, percutaneous absorption effect is not satisfied, the use of liposomes as a carrier can improve its transmission effect, with a good application prospect.
OBJECTIVE: To study the percutaneous penetration of the liposomes carrying low-molecular-weight hyaluronic acid.
METHODS: Low-molecular-weight hyaluronan liposomes were prepared through thin films. Orthogonal experiment was design, and percutaneous penetration was study by snake slough.
RESULTS AND CONCLUSION: The low-molecular-weight hyaluronan liposomes could enwrap more hyaluronic acid when the temperature is 35 ℃, the ratio of cholesterol and lecithin is 0.15∶1, the ratio of hyaluronic acid and lecithin is 0.03∶1, and the pH of hydration medium PBS is 7.5. Hyaluronic acid could penetrate the snake slough more easily when they were enwrapped by liposome, and the percutaneous penetration of hyaluronic acid was improved.

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