中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (42): 7873-7877.doi: 10.3969/j.issn.1673-8225.2010.42.020

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

玉米醇溶蛋白制备牙周组织工程支架材料　　

刘  健，吴景景，李  娜，杨凤英，许彦枝   

  1. 河北医科大学第四医院口腔科，河北省石家庄市 050011
  • 出版日期:2010-10-15 发布日期:2010-10-15
  • 通讯作者: 许彦枝，博士，教授，河北医科大学第四医院口腔科，河北省石家庄市 050011 xu_yanzhi@163.com
  • 作者简介:刘健★，女，1975年生，河北省石家庄市人，汉族，2002年河北医科大学毕业，硕士，主治医师，主要从事牙体牙髓牙周病及口腔黏膜病方面的临床和基础研究。 Zjh888fff@yahoo.cn
  • 基金资助:

    国家自然科学基金面上项目(30873289)，课题名称： 双黄补对牙周组织修复再生作用的研究

Periodontal tissue engineered scaffold materials fabricated with zein

Liu Jian, Wu Jing-jing, Li Na, Yang Feng-ying, Xu Yan-zhi   

  1. Department of Stomatology, the Fourth Hospital of Hebei Medical University, Shijiazhuang   050011, Hebei Province, China
  • Online:2010-10-15 Published:2010-10-15
  • Contact: Xu Yan-zhi, Doctor, Professor, Department of Stomatology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China xu_yanzhi@163.com
  • About author:Liu Jian★, Master, Attending physician, Department of Stomatology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China Zjh888fff@yahoo.cn
  • Supported by:

    General Project of the National Natural Science Foundation of China, No.30873289*

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摘要：

背景：玉米醇溶蛋白具有独特的溶解特性、耐热性、成膜性、抑菌性和抗氧化性，可用于防潮、隔氧、抗紫外线、防静电，已成为医药和生物技术原料的来源。
目的：制备玉米醇溶蛋白支架，探讨该支架应用于牙周组织工程的可行性。
方法：溶剂浇铸/粒子沥滤法制备玉米醇溶蛋白多孔支架，扫描电镜观察支架材料断面的形貌结构和孔隙大小，液体置换法测定支架的孔隙率。采用组织块法培养人牙周膜细胞，并以不同浓度的玉米醇溶蛋白支架浸提液培养，用MTT法测定浸提液培养人牙周膜细胞的活力，计算24，48，72 h的相对增值率并计算毒性分级。将第3代细胞悬液以3.5×107 L-1的密度接种到支架材料上复合培养。
结果与结论：玉米醇溶蛋白支架材料的外观呈一定厚度的高孔隙率海绵体结构。通过控制致孔剂粒子的用量和尺寸可控制支架的孔隙率及孔径，支架孔隙率可达64.1%~78.0%，并具有均匀的互相贯通开放的孔墙结构。玉米醇溶蛋白支架浸提液培养的人牙周膜细胞具有良好的活力，支架毒性程度为0~1级，扫描电镜可见人牙周膜细胞在支架上伸展充分，生长旺盛。提示，溶剂浇铸/粒子沥滤法制备的玉米醇溶蛋白支架具有较高的孔隙率和互相贯通的孔墙结构，具有良好的细胞相容性。

关键词: 玉米醇溶蛋白, 牙周组织工程, 支架, 牙周膜细胞, 细胞相容性

Abstract:

BACKGROUND: Zein with a unique solubility, heat resistance, film-forming, antimicrobial and antioxidant activities, can be used for moisture proofing, oxygen barrier, anti-UV, anti-static electricity. It has become a source of medical and biotechnology raw materials.
OBJECTIVE: To fabricate zein scaffolds and to explore the suitability of zein as scaffolds for periodontal tissue engineering.
METHODS: Zein porous scaffold was made using the solvent casting/particulate leaching method. Scanning electron microscopy was used to observe the fracture surface and the pore size of zein scaffold. The porosity of zein scaffold was measured by liquid displacement method. Human periodontal ligament cells were cultured in vitro using method of tissue piece, then cultured with different concentrations of extracted liquid of zein. MTT assay was used to calculated the relative growth rate of periodontal cells at 24, 48, 72 hours and to evaluate the cytotoxicity. At the third passage, the cell suspension was incubated on zein scaffold at a density of 3.5×107/L.
RESULTS AND CONCLUSIONS: Zein scaffold displayed a sponge-like structure with a high porosity and sufficient thickness. The porosity and pore size of zein scaffold can be controlled by changing the porogen particles dosage and size. Zein porosity was up to 64.1%-78.0% and zein pores were well-distributed, interconnected, and opened. Human periodontal ligament cells cultured with extracted liquid of zein have good activities. The toxicity of zein scaffold was graded as 0-1. Periodontal ligament cells displayed the full stretching and vigorous growth under scanning electronic microscope. All these prompted that zein scaffold fabricated using the solvent casting/particulate leaching method has high porosity, opened pore wall structure, and good cell compatibility.

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