中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (36): 6685-6688.doi: 10.3969/j.issn.1673-8225.2010.36.008

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

脂肪来源干细胞的生物学特性及细胞表型

蔡  震1，潘  博2，林  琳2，蒋海越2，庄洪兴2   

  1. 1四川省医学科学院，四川省人民医院整形外科，四川省成都市   610072；2中国医学科学院北京协和医学院整形外科医院整形七科，北京市 100144
  • 出版日期:2010-09-03 发布日期:2010-09-03
  • 通讯作者: 蒋海越，教授，博士生导师，中国医学科学院北京协和医学院整形外科医院整形七科，北京市 100144 jianghaiyue@ sohu.com
  • 作者简介:蔡震☆，男，1976年生，天津市人，汉族， 2008年中国医学科学院北京协和医学院毕业，博士，主治医师，主要从事组织移植与组织工程研究。 caizhen1976@ 126.com

Biological characteristics and phenotypes of adipose tissue-derived stem cells

Cai Zhen1, Pan Bo2, Lin Lin2, Jiang Hai-yue2, Zhuang Hong-xing2   

  1. 1 Department of Plastic Surgery, Sichuan Academy of Medical Sciences, Sichuan Provincial People’s Hospital, Chengdu  610072, Sichuan Province, China; 2 Seventh Department of Plastic Surgery, Plastic Surgery Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing  100144, China
  • Online:2010-09-03 Published:2010-09-03
  • Contact: Jiang Hai-yue, Professor, Doctoral supervisor, Seventh Department of Plastic Surgery, Plastic Surgery Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100144, China jianghaiyue@sohu. com
  • About author:Cai Zhen☆, Doctor, Attending physician, Department of Plastic Surgery, Sichuan Academy of Medical Sciences, Sichuan Provincial People’s Hospital, Chengdu 610072, Sichuan Province, China caizhen1976@126. com

PDF

806

被引次数

17

摘要：

背景：研究证实脂肪组织提取物中存在脂肪来源的干细胞，并表现出多向分化的潜能，但是关于其体外的生物学特性研究不够深入，其细胞表型的研究结果存在一定争议。
目的：观察脂肪来源的干细胞的生物学特性与细胞免疫表型。
方法：取吸脂手术中废弃的人脂肪组织，胶原酶消化法获得脂肪来源的干细胞，体外传代培养，并进行细胞形态学观察，细胞生长曲线测定，免疫组织化学及流式细胞仪检测其细胞表型。
结果与结论：脂肪来源的干细胞生长旺盛，呈成纤维细胞样生长，15代以内细胞形态未见明显变化；免疫组织化学染色显示，细胞表面标记CD29，CD44，CD105表达阳性，CD34，CD45表达阴性；流式细胞仪检测显示，CD29，CD44，CD105，MHC-Ⅰ为阳性，CD3，CD14，CD19，CD31，CD33，CD34，CD45，CD106，CD117，CD184为阴性。结果说明脂肪来源的干细胞体外生长能力旺盛，高表达干细胞相关抗原，可长期传代且保持低分化状态。

关键词: 表型, 脂肪来源的干细胞, 流式细胞仪, 生物学特性, 干细胞

Abstract:

BACKGROUND: Adipose-derived stem cells (ADSCs) are capable of multi-directional differentiation and exist in human processed lipoaspirate. However, no in-depth studies have addressed biological characteristics of adipose tissue-derived stem cells (ADSCs) in vitro, and there are some disputes on the results from phenotype studies.
OBJECTIVE: To investigate biological characteristics and phenotypes of human ADSCs.
METHODS: Human adipose tissue was collected from persons undergoing fat extraction. The ADSCs were digested with collagenase, cultured and subcultured in vitro. Cell morphology was observed and growth curves were drawn. The phenotypes of ADSCs were identified by immunohistochemistry and flow cytometry.
RESULTS AND CONCLUSION: ADSCs grew well, presented fibroblast-like growth. No significant change in cell morphology was detected within passage 15. The results from immunohistochemistry showed that cells were positive for CD29, CD44, CD105, but negative for CD34, CD45. Flow cytometry results have shown that cells were positive for CD29, CD44, CD105, MHC-Ⅰ, but negative for CD3, CD14, CD19, CD31, CD33, CD34, CD45, CD106, CD117, CD184. Results have suggested that ADSCs can be abundantly harvested and have stable proliferation in poorly differentiated status in vitro. Stem cell-related antigens were highly expressed in ADSCs.

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