中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (36): 6657-6661.doi: 10.3969/j.issn.1673-8225.2010.36.002

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

小鼠骨髓来源早晚期内皮祖细胞的生物学特性

邓正明，孙喜太，朱海涛，丁义涛   

  1. 南京大学医学院附属鼓楼医院，江苏省南京市 210008
  • 出版日期:2010-09-03 发布日期:2010-09-03
  • 通讯作者: 丁义涛，硕士，教授，博士生导师，主任医师，南京大学医学院附属鼓楼医院普通外科，江苏省南京市 210008 yitaoding@hotmail.com
  • 作者简介:邓正明★，男，1981年生，江苏省盐城市人，南京大学医学院在读硕士，主要从事普通外科学方面的研究。 dengzhengming@yahoo.com.cn
  • 基金资助:

    国家自然科学基金项目(30972904)，课题名称：内皮祖细胞在肝癌肝内转移微环境中的作用及分子机理研究；江苏省自然科学基金资助项目(BK2008063)，课题名称：循环血管内皮祖细胞在肝癌诊断与预后判断中的意义。

Biological characteristics of early and late endothelial progenitor cells derived from mouse bone marrow 

Deng Zheng-ming, Sun Xi-tai, Zhu Hai-tao, Ding Yi-tao   

  1. Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing  210008, Jiangsu Province, China
  • Online:2010-09-03 Published:2010-09-03
  • Contact: Ding Yi-tao, Master, Professor, Doctoral supervisor, Chief physician, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, Jiangsu Province, China yitaoding@hotmail.com
  • About author:Deng Zheng-ming★, Studying for master’s degree, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, Jiangsu Province, China dengzhengming@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No.30972904*; the Natural Science Foundation of Jiangsu Province, No. BK2008063*

PDF

512

被引次数

2

摘要：

背景：关于内皮祖细胞统一的定义、相关的生物学特性及在血管发生发展过程中的精准作用等问题存在较大的争议。
目的：探索用序列差速贴壁法分离、培养小鼠骨髓血管早、晚期内皮祖细胞的条件并比较早晚期内皮祖细胞的生物学特性差异。
方法：采用Ficoll 密度梯度离心法从小鼠骨髓中分离单个核细胞，种植于人纤维粘连蛋白包被的培养瓶中，利用序列差速贴壁法分离、纯化细胞早晚期内皮祖细胞。比较早晚期内皮祖细胞在细胞形态、DiI-LDL及FITC-UEA-1摄取能力、细胞的免疫表型、生长增殖能力及体外形成管腔结构能力的差别。
结果与结论：早期内皮祖细胞呈集落样成长，晚期呈铺路石样生长，早、晚期内皮祖细胞都可以吞噬DiI-LDL连接FITC-UEA-1。晚期内皮祖细胞拥有较强增殖潜能，早期在体外基质胶上不形成管腔样结构而晚期内皮祖细胞可形成管腔样结构。提示：骨髓中存在具有不同特性的早晚期内皮祖细胞，晚期内皮祖细胞拥有巨大的增殖潜能并在体外参与血管样结构。

关键词: 内皮祖细胞, 内皮细胞, 骨髓, 成管实验, 血管新生

Abstract:

BACKGROUND: The unified identification, biological characteristics and exact role of endothelial progenitor cells (EPCs) in angiogenesis are controversial.
OBJECTIVE: To explore the conditions which were suitable to isolate and culture the early and late EPCs derived from mouse bone marrow, and compare the different biological characteristics of the early and late EPCs using the method of sequence of differential adhesion.
METHODS: Mononuclear cells were isolated from the mouse bone marrow by Ficoll density gradient centrifugation, and then were grown in culture flask coated fibronectin. The early and late EPCs were isolated and purified using the method of sequence of differential adhesion. We compared the morphology, uptake DiI-LDL and FITC-UEA-1, cell immunophenotype, proliferation ability and capacity to form the cavity structure of the early and late EPCs in vitro.
RESULTS AND CONCLUSION: Early EPCs showed colony-like growth at early time, whereas late EPCs exhibited cobblestone-shaped morphology. Both of the early and late EPCs could phagocytize DiI-LDL and bind to FITC-UEA-1. Late EPCs had greater proliferative potential and formed vascular-like structure whereas early EPCs could not form vascular-like structure. The results suggested that there are early and late EPCs with different characteristics in bone marrow, and late EPCs had greater proliferation potential and formed vascular-like structure.

中图分类号: