中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (33): 6013-6107.doi: 10.3969/j.issn.1673-8225.2010.33.006

• 骨组织构建 bone tissue construction •    下一篇

高糖条件下大鼠下颌骨成骨细胞的活性

吕  娇1，赵文峰1，陈增力1，吴  璇2，刘洪臣3   

  1. 1解放军北京军区总医院口腔科，北京市  100700；  2大连市口腔医院口腔修复科，辽宁省大连市  116021；3解放军总医院口腔医学研究所，北京市  100853
  • 出版日期:2010-08-13 发布日期:2010-08-13
  • 通讯作者: 刘洪臣，博士，教授，解放军总医院口腔医学研究所，北京市 100853 liu_hc@301dent.com
  • 作者简介:吕 娇☆，女，1977年生，辽宁省大连市人，汉族，2008年解放军军医进修学院毕业，博士，医师，主要从事糖尿病与牙种植方面的基础研究。

Effect of hyperglycemia on activity of rat mandibular osteoblasts

Lü Jiao1, Zhao Wen-feng1, Chen Zeng-li1, Wu Xuan2, Liu Hong-chen3   

  1. 1 Department of Stomatology, General Hospital of Beijing Military Area Command of Chinese PLA, Beijing  100700, China; 2 Department of Stomatology, Dalian Stomatologic Hospital, Dalian 116021, Liaoning Province, China; 3 Stomatologic Research Institute of General Hospital of Chinese PLA, Beijing  100853, China
  • Online:2010-08-13 Published:2010-08-13
  • Contact: Liu Hong-chen, Doctor, Professor, Stomatologic Research Institute of General Hospital of Chinese PLA, Beijing 100853, China liu_hc@301dent.com
  • About author:Lü Jiao☆, Doctor, Physician, Department of Stomatology, General Hospital of Beijing Military Area Command of Chinese PLA, Beijing 100700, China

PDF

346

被引次数

11

摘要：

背景：临床研究证实，糖尿病是引起牙周病变及牙槽骨丧失的危险因素，而糖尿病所致的高血糖对颌骨改变的影响机制目前仍未完全阐明。
目的：观察高糖对体外培养的下颌骨成骨细胞增殖、分化、矿化的影响。
方法：分离培养大鼠下颌骨成骨细胞，分别在生理糖浓度(5.5 mmol/L；对照组)和高糖条件下(16.5 mmol/L；高糖组)培养，采用MTT法、PNPP法、生化法、放射免疫法及茜素红S钙染法检测各组细胞的增殖能力、碱性磷酸酶活性、钙吸收、骨钙素分泌水平及矿化能力。
结果与结论：高浓度的葡萄糖能显著促进成骨细胞的增殖(P < 0.05)，降低成骨细胞骨钙素的分泌(P < 0.01)，增加钙结节的数量及面积(P < 0.01)。与对照组比较，高糖组成骨细胞碱性磷酸酶活性在21 d时显著增高(P < 0.01)，钙吸收在14~21 d时显著降低，而在21~28 d时显著增高(P < 0.01)。说明高糖能促进体外培养的大鼠下颌骨成骨细胞的增殖，延迟其分化和矿化。

关键词: 葡萄糖, 成骨细胞, 糖尿病, 下颌骨, 大鼠

Abstract:

BACKGROUND: Clinical research has demonstrated that diabetes mellitus is a risk factor for periodontal lesion and alveolar bone loss. However, it remains poorly understood the effect of hyperglycemia on changes of jaw bone.
OBJECTIVE: To investigate the effects of hyglycemia on proliferation, differentiation and mineralization of osteoblasts from rat mandible.
METHODS: Primary osteoblasts were isolated and incubated with medium containing 5.5 mmol/L and 16.5 mmol/L glucose, respectively. Cell proliferation, alkaline phosphatase (ALP) activity, calcium uptake, bone gla protein (BGP) and mineralization were detected using MTT, PNPP, biochemistry, radioimmunoassay, as well as Alizarin Red S staining.
RESULTS AND CONCLUSION: High glucose could significantly increase cell proliferation (P < 0.05), decreased BGP level (P < 0.01), and increase number and the area of nodules (P < 0.01). Compared with the control group, the ALP activity of the high glucose group was obviously increased at 21 days after culture (P < 0.01); the calcium uptake was decreased at 14-21 days, but notably increased at 21-28 days (P < 0.01). These findings suggest that diabetes-associated hyperglycemia promotes rat mandibular osteoblasts proliferation while delaying differentiation and mineralization.

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