中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (31): 5749-5752.doi: 10.3969/j.issn.1673-8225.2010.31.013

• 细胞与组织移植 cell and tissue transplantation • 上一篇    下一篇

青年供体胰段移植的超微结构及解剖学基础

叶维建1，郑玉涛1，蔡兆明2   

  1. 1莆田学院人体解剖学教研室，福建省莆田市    351100；2福建医科大学人体解剖学教研室，福建省福州市     350004
  • 出版日期:2010-07-30 发布日期:2010-07-30
  • 基金资助:

    福建省教育厅基金项目(JB06157)；莆田市科学技术局基金项目(2005S08)；莆田学院基金项目(2005057)。课题名称：胰腺移植的超微结构和应用解剖研究。

Ultra-structure and anatomical basis of pancreatic segmental transplantation from young donors

Ye Wei-jian 1, Zheng Yu-tao1, Cai Zhao-ming2   

  1. 1 Department of Anatomy, Putian College, Putian  351100, Fujian Province, China; 2 Department of Anatomy, Fujian Medical University, Fuzhou  350004, Fujian Province, China
  • Online:2010-07-30 Published:2010-07-30
  • Supported by:

    the Foundation of Education Department of Fujian Province, No. JB06157*; the Foundation of Science and Technology Bureau of Putian, No. 2005S08*; the Foundation of Putian College, No. 2005057*

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摘要：

背景：在胰腺和胰岛移植中，青年供体胰腺血管吻合难度较小，成功概率大，但目前青年供体胰腺胰段移植的解剖学基础国内尚缺乏。
目的：观察青年人胰腺胰尾的微细结构、胰岛B细胞胰高血糖素和胰岛素阳性表达面积及胰岛B细胞免疫阳性染色面积，以及青年尸体胰腺血管的解剖情况。
方法：选择青年人尸体12例，取胰尾切片苏木精-伊红染色后光镜观察，显微测微器测量胰岛数量和面积，用胰高血糖素抗血清和胰岛素抗血清进行免疫组织化学染色。另取3例青年人尸体取胰尾组织块铀铅染色。透射电镜观察青年供体胰腺B细胞，胰岛形态和数量、结构及其分泌颗粒，以及青年供体胰腺血管情况。
结果与结论：青年供体胰尾组织中胰岛B细胞内分泌颗粒多，内质网、高尔基复合体和线粒体等细胞器发达，胰岛数目分布多，胰岛B细胞的免疫阳性染色面积大。青年供体胰尾有较多而密的胰岛B细胞散在分布，胰岛B细胞分泌颗粒多。血管适合做端侧吻合。提示青年供体胰腺细胞的分泌活动旺盛，血管适合做端侧吻合，适宜作为胰腺胰段胰岛移植供体。

关键词: 胰段移植, 青年胰腺, 超微结构, 免疫组化, 解剖

Abstract:

BACKGROUND: The success probability of young donors for pancreatic or islet transplantation is great due to easy vascular anastomosis. However, the anatomical basis of young donors for pancreatic segmental transplantation are insufficient.
OBJECTIVE: To observe the microstructure, positive expression areas of pancreatic islet B cell glucagons and insulin, and immune positive staining area of pancreatic islet B cells in the pancreatic tails of young people, and to explore the pancreatic vascular anatomy of young cadavers.
METHODS: Totally 12 cases of young cadavers were randomly selected. Their pancreatic tails sections were treated by hematoxylin-eosin staining and observed under an optic microscope. The number and area of pancreatic islets were measured with micrometer gauge. And the immunohistochemical staining was done with pancreatic glucagons antiserum and insulin antiserum. Pancreatic tails of another 3 young cadavers were stained with uranium and lead. The pancreatic islet B cells, the morphology, the quantity and the secreting granules and the vascular situation of the young donors were observed.
RESULTS AND CONCLUSION: In the tissues of the young donors, the secreting granules in pancreatic islet B cells were abundant, organelles such as the endoplasmic reticula, the Golgi complex and the mitochondria were mature and advanced, the insulin islets were widely distributed, and the immune staining positive expression area of the insulin B cells was large. There were many and dense insulin B cells distributed at the pancreatic tails of the young donors, and there were many secreting granules in the insulin islets B cells. The blood vessels were suitable for end-to-side anastomosis. Since the secreting activities of pancreatic cells of the young donors were vigorous and the blood vessels were suitable for end-to-side anastomosis, the young donors are suitable for pancreatic segmental insulin islets transplantation.

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