中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (21): 3847-3850.doi: 10.3969/j.issn.1673-8225.2010.21.011

• 药物控释材料 drug delivery materials • 上一篇    下一篇

壳聚糖和葡甘聚糖生物膜制备及体外性能

段开文1，赵利芬2，李承文3，陈庆华4，马丽丽1，李娜娜4   

  1. 1昆明医学院附属延安医院口腔科，云南省昆明市 650051；2昆明医学院海源学院，云南省昆明市  650106；3云南高等医学专科学校，云南省昆明市  650021；4昆明理工大学材料与冶金工程学院，云南省昆明市  650093
  • 出版日期:2010-05-21 发布日期:2010-05-21
  • 作者简介:段开文☆，男，1965年生，云南省玉溪市人，汉族， 1997年华西医科大学毕业，博士，教授，主要从事牙周病的病因与防治研究。 kwduan@yahoo.com.cn

Preparation of chitosan/konjac glucomannan composite membranes and the in vitro performances

Duan Kai-wen1, Zhao Li-fen2, Li Cheng-wen3, Chen Qing-hua4, Ma Li-li1, Li Na-na4   

  1. 1 Department of Stomatology, Affiliated Yan’an Hospital of Kunming Medical University, Kunming  650051, Yunnan Province, China; 2 Haiyuan School of Kunming Medical College, Kunming  650021, Yunnan Province, China; 3 Yunnan Medical College, Kunming  650021, Yunnan Province, China; 4 College of Material and Metallurgical Engineering, Kunming University of Science and Technology, Kunming  650093, Yunnan Province, China
  • Online:2010-05-21 Published:2010-05-21
  • About author:Duan Kai-wen☆, Doctor, Professor, Department of Stomatology, Affiliated Yan’an Hospital of Kunming Medical University, Kunming 　650051, Yunnan Province, China kwduan@yahoo.com.cn

PDF

438

被引次数

8

摘要：

背景：近年来，不少学者发现将壳聚糖和葡甘聚糖共混，可改善二者单独使用某些性能的不足。
目的：制备壳聚糖和葡甘聚糖复合生物膜，并评价其对人牙周膜细胞增殖的影响。
方法：将壳聚糖与葡甘聚糖以不同质量比混合，采用流延法制备壳聚糖与葡甘聚糖混合膜，与第6代人脐带间充质干细胞培养评价细胞相容性和细胞毒性，测定其体内外降解性能。并观察混合膜对人牙周膜细胞的作用。
结果与结论：获得壳聚糖/葡甘聚糖含量分别为3∶0，2∶1，1∶1，1∶2 的8种良好组织相容性膜。体外降解实验显示，随着葡甘聚糖含量增加，壳聚糖/葡甘聚糖膜的降解率提高。在体外有溶菌酶存在时，混合膜70 d降解率分别为40.75%、65.70%，74.20%和84.60%；体内降解实验显示，壳聚糖/葡甘聚糖含量比为3∶0，2∶1，1∶1时在体内可存留12周以上。当葡甘聚糖含量占主要成分如壳聚糖/葡甘聚糖比为1∶2时，可在体内存留8周。随着葡甘聚糖含量的增加，壳聚糖/葡甘聚糖混合膜对人牙周膜细胞增殖作用逐渐减弱，加载碱性成纤维细胞生长因子后，壳聚糖/葡甘聚糖含量比为3∶0，2∶1的混合膜可提高细胞碱性磷酸酶活性，但以2∶1的混合膜最明显。结果说明所获得的混合膜初步具备了诱导组织再生膜和组织工程支架的基本性能。

关键词: 壳聚糖, 葡甘聚糖, 混合膜, 引导组织再生, 牙周膜细胞, 生物材料

Abstract:

BACKGROUND: Recent studies indicated that the composition of chitosan (CS) and konjac glucomannan (KGM) can improve their own biological features.
OBJECTIVE: To prepare CS / KGM composite membranes, and to evaluate its effect on the proliferation of periodontal ligament fibroblast cells (PDLCs).
METHODS: Blend membranes containing CS and KGM solutions with different weight proportions were prepared by solvent-casting method. The 6th passage of human umbilical cord mesenchymal stem cells were used to observe the cell compatibility and cytotoxicity and to determine the degradation features. The effects of those cells on PDLCs were assessed.
RESULTS AND CONCLUSION: Eight kinds of composite membranes containing CS and KGM (3:0, 2:1, 1:1, 1:2) were obtained. In vitro degradation test demonstrated that the degradation rates of CS / KGM composite membranes were increased with the adding of KGM, which were 40.75%, 65.70%, 74.20% and 84.60% respectively at 70 days. In vivo test showed that the CS / KGM composite membranes could maintain more than 12 weeks when at the CS / KGM ratios of 3:0, 2:1 and 1:1. If the CS / KGM ratio was 1:2, the CS / KGM composite membranes could maintain 8 weeks in vivo. The effects of the composite membranes on human PDLCs were decreased with increasing of KGM contents. After adding basic fibroblast growth factor, the CS / KGM composite membranes with ratios of 3:2 and 2:1 could increase the alkaline phosphatase activities, especially with ratio of 2:1. Results from the study suggested that CS / KGM membrane is a potential periodontal tissue engineering scaffold.

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