中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (19): 3554-3557.doi: 10.3969/j.issn.1673-8225.2010.19.027

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

吡咯喹啉醌对许旺细胞增殖及Sox10表达的影响

贺 斌，刘世清，李皓桓   

  1. 武汉大学人民医院骨科，湖北省武汉市 430060
  • 出版日期:2010-05-07 发布日期:2010-05-07
  • 通讯作者: 刘世清，教授，博士生导师，武汉大学人民医院骨科，湖北省武汉市 430060 liu_shiqing2009@163.com
  • 作者简介:贺 斌，男，1983年生，湖北省武汉市人，汉族，武汉大学在读博士，主要从事脊髓损伤与骨关节病的研究。 hebin_0203@163.com
  • 基金资助:

    国家自然科学基金资助项目(30600627)

Effects of pyrroloquinoline quinone on Schwann cells proliferation and Sox10 expression

He Bin, Liu Shi-qing, Li Hao-huan   

  1. Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
  • Online:2010-05-07 Published:2010-05-07
  • Contact: Liu Shi-qing, Professor, Doctoral supervisor, Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China liu_shiqing2009@163. com
  • About author:He Bin, Studying for doctorate, Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China hebin_0203@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30600627*

PDF

425

被引次数

7

摘要：

背景：许旺细胞是神经组织工程的种子细胞，但其体外增殖缓慢，难以满足科研与临床对其的需要，而吡咯喹啉醌可以对多种细胞的增殖产生促进作用。

目的：观察吡咯喹啉醌对许旺细胞的增殖作用并探讨其对许旺细胞Sox10基因表达的影响。

方法：体外培养及纯化大鼠许旺细胞，S-100免疫荧光鉴定许旺细胞；细胞经无血清培养12 h后，加入10 nmol/L吡咯喹啉醌继续培养24 h观察其形态学改变；加入不同浓度(0，1，10，100，1 000，10 000 nmol/L)吡咯喹啉醌于许旺细胞培养24 h，利用RT-PCR技术检测Sox10的mRNA表达。

结果与结论：吡咯喹啉醌促使许旺细胞发生形态学改变，多数呈束状或并排生长，且细胞数目增多；1~1 000 nmol/L吡咯喹啉醌可使许旺细胞Sox10 mRNA表达增高，100 nmol/L时表达最高；10 000 nmol/L时对Sox10的表达表现为抑制作用(P < 0.05)。

关键词: 吡咯喹啉醌, 许旺细胞, 增殖, Sox10, 大鼠

Abstract:

BACKGROUND: Schwann cells are seed cells of nerve tissue engineering, but they grow slowly in vitro, and can not satisfy the need of scientific research and clinical application. Pyrroloquinoline quinine (PQQ) can promote proliferation of some kinds of cells.

OBJECTIVE: To investigate the effects of PQQ on Schwann cells proliferation and Sox10 expression.

METHODS: Schwann cells were cultured and purified in vitro. The purity of Schwann cells was identified by immunofluorescence of S-100. After serum-free culture for 12 hours, 10 nmol/L PQQ was added into cultured Schwann cells, and the morphological changes were detected under PQQ treated. Different concentrations of PQQ (0, 1, 10, 100, 1 000, 10 000 nmol/L) were added into culture medium for 24 hours, then the expression of Sox10 mRNA was detected by reverse transcription-polymerase chain reaction.

RESULTS AND CONCLUSION: PQQ could affect the morphology of Schwann cells, showing bundle-shaped and side by side growing, and promote Schwann cell proliferation. 1-1 000 nmol/L PQQ could up-regulate the expression of Sox10 mRNA on cultured Schwann cells; the maximal effect occurred on 100 nmol/L PQQ; 10 000 nmol/L PQQ exhibited the depressed effect on expression of Sox10 on cultured Schwann cells (P < 0.05).

中图分类号: