中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (19): 3518-3522.doi: 10.3969/j.issn.1673-8225.2010.19.020

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

淫羊藿苷对大鼠间充质干细胞骨向分化过程中转化生长因子β1、骨形态发生蛋白2表达的影响

杨 丽1，张荣华1，朱晓峰2，蔡 宇1，黄 丰3   

  1. 暨南大学药学院，1中药学教研室， 3药理学教研室，广东省广州市  510632；
    2暨南大学附属第一医院中医科，广东省广州市  510632；
  • 出版日期:2010-05-07 发布日期:2010-05-07
  • 作者简介:杨 丽☆，，1979年生，湖北省荆门市人，汉族，2007年广州暨南大学毕业，博士，讲师，主要从事骨质疏松症及干细胞的研究。 doctormonkey@126.com
  • 基金资助:

    国家自然科学基金资助项目(30472274)；
    广东省自然科学基金重点资助项目(04105837)；
    广东省社会发展计划重点引导项目(2004B33001004)

Effect of icariin on the expression of transforming growth factor-beta 1 and bone morphogenetic protein-2 in the process of mesenchymal stem cells differentiation into osteoblasts

Yang Li1, Zhang Rong-hua1, Zhu Xiao-feng2, Cai Yu1, Huang Feng3   

  1. 1Staff Room of Traditional Chinese Medicine, School of Pharmacy, Jinan University, Guangzhou  510632, Guangdong Province, China;
    2Department of Traditional Chinese Medicine, First Affiliated Hospital, Jinan University, Guangzhou  510632, Guangdong Province, China;
    3Staff Room of Pharmacology, School of Pharmacy, Jinan University, Guangzhou  510632, Guangdong Province, China
  • Online:2010-05-07 Published:2010-05-07
  • About author:Yang Li, Doctor, Lecturer, Staff Room of Traditional Chinese Medicine, School of Pharmacy, Jinan University, Guangzhou 510632, Guangdong Province, China doctormonkey@126.com
  • Supported by:

    the National Nature Science Foundation of China, No. 30472274*;
    a Grant from Natural Science Foundation of Guangdong Province, No. 04105837*;
    an Accented and Lead Term from Social Development Project of Guangdong Province, No. 2004B33001004*

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摘要：

背景：淫羊藿苷作为补肾中药淫羊藿的主要有效成分，能够有效促进间充质干细胞向成骨细胞分化。

目的：观察淫羊藿苷在诱导间充质干细胞骨向分化过程中对转化生长因子β1、骨形态发生蛋白2表达的影响，阐释其可能的诱导机制。

方法：运用全骨髓贴壁法分离、纯化大鼠骨髓间充质干细胞；以20 mg/L淫羊藿苷作为间充质干细胞药物干预浓度，根据干预条件的不同将实验分为：空白组、经典组(加经典成骨诱导剂)、淫羊藿苷组、淫羊藿苷+经组；ELISA法检测各组转化生长因子β1、骨形态发生蛋白2的表达。

结果与结论：经典组、淫羊藿苷组、淫羊藿苷+经组转化生长因子β1、骨形态发生蛋白2值均高于空白组(P < 0.05)；各组第7天的转化生长因子β1值高于14，21 d (P < 0.01)，各组不同诱导时间骨形态发生蛋白2值之间相比差异无显著性意义(P > 0.05)。提示上调转化生长因子β1、骨形态发生蛋白2的表达量可能是淫羊藿苷诱导各组促进间充质干细胞骨向分化的作用机制之一。

关键词: 淫羊藿苷, 骨髓间充质干细胞, 大鼠, 转化生长因子β1, 骨形态发生蛋白2

Abstract:

BACKGROUND: As the main effective ingredient of epimedium, icariin (ICA) can promote the differentiation of mesenchymal stem cells (MSCs) into osteoblasts.

OBJECTIVE: To observe effects of ICA on the expression of transforming growth factor-β1 (TGF-β1) and bone morphogenetic protein-2 (BMP-2) in the process of mesenchymal stem cells differentiation into osteoblasts, and to explain the inducing mechanisms.  

METHODS: MSCs were isolated and purified by whole marrow adherent method. 20 μg/mL ICA was used to be the intervention concentration; according to the different induced conditions, MSCs were divided into 4 groups: blank group, classic group (induced by the classic osteoblast-induced system), ICA group (induced by 20 μg/mL ICA) and classic+ICA group (induced by the combination of classic osteoblast-induced system and 20 μg/mL ICA). ELISA was used to detect the expression of TGF-β1 and BMP-2 of each induced group in the progress of MSCs differenting into osteoblasts.

RESULTS AND CONCLUSION: The expression of TGF-β1 and BMP-2 of the classic group, ICA group and classic+ICA group were all higher than that of the blank group (P < 0.05); the expression of TGF-β1 of each group was higher at 7 days than that at 14 and 21 days (P < 0.01); however, the expression of BMP-2 of each group at 7, 14 and 21 days had no statistical difference (P > 0.05). The results revealed that upregulation of the expression of TGF-β1 and BMP-2 in the ICA-induced groups may be the mechanisms on improving the differentiation of MSCs into osteoblasts.

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