中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (19): 3495-3498.doi: 10.3969/j.issn.1673-8225.2010.19.015

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

辣根过氧化物酶逆行示踪评价无细胞神经移植物复合骨髓间充质干细胞对坐骨神经缺损大鼠运动神经元的作用

赵  硕，张彩顺   

  1. 辽宁医学院，辽宁省锦州市   121001
  • 出版日期:2010-05-07 发布日期:2010-05-07
  • 通讯作者: 张彩顺，副教授，辽宁医学院附属第一医院手外科，辽宁省锦州市 121001 zcs731009@163.com
  • 作者简介:赵 硕，女，1982年生，辽宁省锦州市人，锡伯族，辽宁医学院附属第一医院病理科在读硕士，主要从事周围神经组织工程方面的研究。
  • 基金资助:

    辽宁省教育厅高校创新团队项目(2008T114)

Effects of cell-free nerve graft compounded with bone marrow mesenchymal stem cells on motor neurons of sciatic nerve defect rats: An evaluation using horseradish peroxidase retrograde tracer technique

Zhao Shuo, Zhang Cai-shun   

  1. Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
  • Online:2010-05-07 Published:2010-05-07
  • Contact: Zhang Cai-shun, Associate professor, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China zcs731009@163.com
  • About author:Zhao Shuo, Studying for master’s degree, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
  • Supported by:

    the Innovation Team Program for Higher Learning School of Department of Education of Liaoning Province, No. 2008T114*

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被引次数

1

摘要：

背景：作者前期将无细胞神经移植物与骨髓间充质干细胞复合培养，成功构建了组织工程人工神经。
目的：应用辣根过氧化物酶(HRP)神经逆行示踪技术对无细胞神经移植物复合骨髓间充质干细胞构建的神经移植复合体桥接大鼠坐骨神经缺损后运动神经元的保护作用进行评价。
方法：成年清洁级健康雄性SD大鼠，随机分成3组：①实验组：采用复合骨髓间充质干细胞的无细胞神经移植物桥接大鼠坐骨神经缺损。②空白对照组：采用无细胞神经移植物桥接大鼠坐骨神经缺损。③自体神经对照组：采用自体神经移植桥接大鼠坐骨神经缺损。术后12周应用辣根过氧化物酶神经逆行示踪技术对脊髓前角运动神经元的再生进行评价。
结果与结论：术后12周脊髓前角运动神经元再生评价结果显示：实验组优于无细胞神经移植物组，而与自体神经移植物组相比差异无显著性意义。证实无细胞神经移植物复合骨髓间充质干细胞构建组织工程人工神经修复大鼠坐骨神经缺损，对大鼠脊髓运动神经元具有保护作用，可能达到与自体神经移植相似的效果。

关键词: 无细胞神经移植物, 骨髓间充质干细胞, 辣根过氧化物酶, 神经移植v大鼠

Abstract:

BACKGROUND: The authors have done compound culture of cell-free nerve graft and bone marrow mesenchymal stem cells (BMSCs), and successfully constructed tissue-engineered artificial nerves.
OBJECTIVE: Horseradish peroxidase (HRP) nerve retrograde tracer technique was used to evaluate protective effects on motor neurons following sciatic nerve defect bridging with neural transplantation complex constructed by cell-free nerve graft and BMSCs in rats.
METHODS: Adult clean healthy male Sprague Dawley rats were randomly assigned to three groups: (1) experimental group: rat sciatic nerve detect was bridged by cell-free nerve graft combined with BMSCs; (2) blank control group: rat sciatic nerve defect was bridged by cell-free nerve graft; (3) autologous nerve control group: rat sciatic nerve defect was bridged by autologous nerve transplantation. Regeneration of motor neurons in the spinal cord anterior horn was assessed using HRP nerve retrograde tracer technique at 12 weeks following surgery.
RESULTS AND CONCLUSION: Motor neuron regeneration in the spinal cord anterior horn at 12 weeks following surgery was better in the experimental group compared with cell-free nerve graft group. No significant difference was detected as compared with autologous nerve graft. These findings indicate that tissue-engineered artificial nerves constructed by cell-free nerve graft and BMSCs have protective effects on spinal cord motor neurons, and can obtain a similar effect to autologous neural transplantation in repair of rat sciatic nerve defect.

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