中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (19): 3483-3489.doi: 10.3969/j.issn.1673-8225.2010.19.013

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

移植人脐带间充质干细胞修复大鼠脊髓损伤

韩明远，冯世庆，李  辉，王春源，于铁强   

  1. 天津医科大学总医院骨科，天津市  300052
  • 出版日期:2010-05-07 发布日期:2010-05-07
  • 通讯作者: 冯世庆，主任医师，天津医科大学总医院骨科，天津市 300052
  • 作者简介:韩明远，男，1982年生，河北省迁西县人，汉族，天津医科大学总医院骨科在读硕士，医师，主要从事脊柱脊髓损伤及关节镜的研究。 mingyuanhan@yahoo.com.cn

Transplantation of human umbilical cord-derived mesenchymal stem cells for the treatment of spinal cord injury in rats

Han Ming-yuan, Feng Shi-qing, Li Hui, Wang Chun-yuan, Yu Tie-qiang   

  1. Department of Orthopaedics, General Hospital, Tianjin Medical University, Tianjin  300052, China
  • Online:2010-05-07 Published:2010-05-07
  • Contact: Feng Shi-qing, Chief physician, Department of Orthopaedics, General Hospital, Tianjin Medical University, Tianjin 300052, China
  • About author:Han Ming-yuan, Studying for master’s degree, Physician, Department of Orthopaedics, General Hospital, Tianjin Medical University, Tianjin 300052, China mingyuanhan@yahoo.com.cn
  • Supported by:

    the Doctor Center Program of Ministry of Education, No. 200800620003*;
    the Science and Technology Support Project of Committee of Science and Technology of Tianjin City, No. 08ZCKFSF03200*;
    the Scientific Research Foundation of Bureau of Health of Tianjin City, No. 06K243* 

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503

被引次数

36

摘要：

背景：已知人脐带间充质干细胞对脊髓损伤存在着潜在的治疗价值，然而，当前对移植人脐带间充质干细胞治疗脊髓损伤及机制方面研究很少。

目的：观察人脐带间充质干细胞对脊髓损伤大鼠的治疗效果。

方法：40只Wistar大鼠建立脊髓损伤模型，38只造模成功后随机摸球法分为3组：空白对照组：只接受单纯损伤，不做任何移植；DMEM移植组：损伤后1周予以5 μL DMEM局部移植；细胞移植组：损伤后1周予以5 μL准备好的人脐带间充质干细胞局部移植(细胞数1×106)。移植后对实验动物通过BBB评分、体感诱发电位与运动诱发电位观察后肢功能恢复情况。分别于损伤后2，4，6，8，10周随机于细胞移植组抽取大鼠2只，免疫组织化学染色观察人脐带间充质干细胞存活、迁移、分化，通过胶质纤维酸性蛋白阳性细胞染色比较各组损伤局部胶质瘢痕形成面积。

结果与结论：BBB评分损伤后4周细胞移植组高于其他两组(P < 0.05)，损伤后12周细胞移植组与其他两组相比SEP、MEP潜伏期缩短、波幅值增高(P < 0.05)。免疫组织化学染色示人脐带间充质干细胞可向神经元、星形胶质细胞和少突胶质细胞分化，分化的少突胶质细胞并包绕轴突形成髓鞘。细胞移植组损伤局部胶质瘢痕面积均小于其他两组(P < 0.05)，空白对照组、DMEM移植组间差异无显著性(P > 0.05)。提示未经体外诱导的人脐带间充质干细胞可于损伤大鼠脊髓体内向神经元、星形胶质细胞、少突胶质细胞分化，减小胶质瘢痕，并促进脊髓损伤大鼠神经功能的恢复。

关键词: 脐带间充质干细胞, 细胞移植, 脊髓损伤, 局部移植, 损伤修复

Abstract:

BACKGROUND: Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) have the potential therapeutic value of repairing the injured spinal cord. But, there are rare studies concerning the mechanisms of repairing spinal cord injury by transplanting hUC-MSCs.

OBJECTIVE: To observe therapeutic effects of hUC-MSCs on rats with spinal cord injury.

METHODS: A total of 40 Wistar rats were selected to establish models of spinal cord injury. Following successful establishment, 38 rat models were assigned to three groups randomly: blank control group: only received simple injury, no transplantation; DMEM transplantation group: subjected to local transplantation of 5 μL DMEM at 1 week following injury; cell transplantation group: underwent 5 μL hUC-MSCs via local injection at 1 week following injury (1×106 cells). Basso-Beattie-Bresnahan (BBB) locomotor scoring system, somatosensory evoked potential and motor evoked potentials (SEP&MEP) were used to observe the recovery of hindlimb function following transplantation. Two rats were randomly selected from cell transplantation group at 2, 4, 6, 8 and 10 weeks following injury. Survival, migration and differentiation of hUC-MSCs in the injury site were observed by immunohistochemistry. The area of glial scar in the injury site was calculated by immunostaining against glial fibrillary acidic protein (GFAP).

RESULTS AND CONCLUSION:BBB score was higher in the cell transplantation group than in other two groups at 4 weeks following injury (P < 0.05). Latency period and wave amplitude in SEP&MEP were increased in cell transplantation group compared with another two groups (P < 0.05). Immunohistochemical staining showed hUC-MSCs could differentiate into neurons, oligodendrocytes and astrocytes. Differentiated oligodendrocytes surrounded axons and formed myelin sheath. The area of glial scar in cell transplantation group was smaller than in the blank control and DMEM transplantation groups (P < 0.05). There were no significant differences between blank control and DMEM transplantation groups (P > 0.05). Results have indicated that hUC-MSCs transplanted in the injured spinal cord can differentiate into neurons, oligodendrocytes and astrocytes, reduce glial scar and improve the recovery of neural function of rats with spinal cord injury

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