中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (18): 3314-3317.doi: 10.3969/j.issn.1673-8225.2010.18.020

• 移植与免疫 transplantation and Immunology • 上一篇    下一篇

不同免疫抑制剂对全血细胞单核细胞趋化蛋白1分泌的影响

王  明，何  懿，刘占国，罗宇维，武  凯，孙尔维，赵  明   

  1. 南方医科大学珠江医院器官移植科，广东省广州市  510282
  • 出版日期:2010-04-30 发布日期:2010-04-30
  • 通讯作者: 赵 明，教授，主任医师，博士生导师，南方医科大学珠江医院器官移植科，广东省广州市 510282； 通讯作者：孙尔维，主任医师，博士生导师，南方医科大学珠江医院器官移植科移植免疫研究所，广东省广州市 510282
  • 作者简介:王 明★，男，1983年生，宁夏回族自治区青铜峡市人，汉族，南方医科大学在读硕士，主要从事移植免疫研究。 wlzlm2010@163.com

Influence of different immunosuppressants on monocyte chemoattractant protein-1 secretions in the whole blood

Wang Ming, He Yi, Liu Zhan-guo, Luo Yu-wei, Wu Kai, Sun Er-wei, Zhao Ming   

  1. Department of Organ Transplantation, Zhujiang Hospital of Southern Medical University, Guangzhou   510282, Guangdong Province, China
  • Online:2010-04-30 Published:2010-04-30
  • Contact: Zhao Ming, Professor, Chief physician, Doctoral supervisor, Department of Organ Transplantation, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China Sun Er-wei, Chief physician, Doctoral supervisor, Department of Organ Transplantation, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China
  • About author:Wang Ming★, Studying for master’s degree, Department of Organ Transplantation, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China wlzlm2010@163.com

PDF

441

被引次数

6

摘要：

背景：器官移植后的患者常规使用地塞米松、他克莫司、环孢素A和麦考酚酸等临床常用的免疫抑制剂，其作用机制各不相同。有关不同免疫抑制剂对单核细胞趋化蛋白1的作用尚未见报道。
目的：观察不同免疫抑制剂对全血细胞分泌单核细胞趋化蛋白1的影响。
方法：取健康志愿者全血与不同免疫抑制剂(他克莫司，环孢素A，地塞米松，麦考酚酸，每种免疫抑制剂又分为低、中、高3个质量浓度)作用6 h后，用佛波酯联合离子霉素刺激6 h，比较不同免疫抑制剂组及免疫抑制剂联合使用单核细胞趋化蛋白1的水平。以全血单独培养作为对照。
结果与结论：麦考酚酸和他克莫司不能有效抑制单核细胞趋化蛋白1的分泌；1，10 mg/L地塞米松、0.05，1.25 mg/L环孢素A均能够显著抑制单核细胞趋化蛋白1的分泌。因此，地塞米松和环孢素A可能通过抑制单核细胞趋化蛋白1的水平抑制单核巨噬细胞在免疫系统中发挥作用的。联合使用5 µg/L他克莫司、10 mg/L麦考酚酸和1 mg/L地塞米松或者联合使用0.25 mg/L环孢素A、10 mg/L麦考酚酸和1 mg/L地塞米松都可以有效抑制单核细胞趋化蛋白1的分泌，而上述免疫抑制剂单用时，仅地塞米松能有效抑制单核细胞趋化蛋白1的分泌。

关键词: 免疫抑制剂, 单核细胞趋化蛋白1, 全血, 刺激, 器官移植

Abstract:

BACKGROUND: In the field of organ transplantation, patients often take immunosuppressants after organ transplantation, such as CsA, FK506, DEX and MPA. However, their mechanisms of immunosuppression are different. The effect of immunosuppressive drugs on monocyte chemoattractant protein-1 (MCP-1) remains poorly understood.
OBJECTIVE: To investigate the effects of different immunosuppressants on the secretions of MCP-1 in whole blood.
METHODS: The whole blood of healthy volunteers was mixed with different immunosuppressants for 6 hours, such as CsA, FK506, DEX and MPA, which included low, middle and high concentrations, followed by PMA and IONO stimulation for 6 hours. MCP-1 levels in whole blood samples were compared. The whole blood cultured alone served as control.
RESULTS AND CONCLUSION: MCP-1 secretion was inhibited by DEX (1, 10 mg/L) and CsA (0.25, 1.25 mg/L). However, FK and MPA exhibited no such effect. Therefore, DEX and CsA may inhibit the function of monocytes and macrophages in immune system by diminishing the secretion of MCP-1. The combination of FK (5 µg/L), MPA (10 mg/L) and DEX (1mg/L) or CsA    (0.25 mg/L), MPA (10 mg/L) and DEX (1 mg/L) can inhibit the secretion of MCP-1, but only DEX among all the immunosuppressants mentioned above exhibited significant effect on inhibiting the secretion of MCP-1 when using alone.

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