中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (16): 2903-2906.doi: 10.3969/j.issn.1673-8225.2010.16.014

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

比较电纺β-磷酸三钙/明胶引导组织再生膜与聚乳酸、聚丙交酯/乙交酯膜的生物相容性

郑钧元1，唐  明1，邓旭亮2，杨小平3，张  慎3，李晓霞1   

  1. 1重庆医科大学附属第一医院口腔科，重庆市 400016；2北京大学口腔医学院，北京市 100081；3北京化工大学北京市新型高分子材料制备与加工重点实验室，北京市100029
  • 出版日期:2010-04-16 发布日期:2010-04-16
  • 通讯作者: 唐 明，硕士，副教授，重庆医科大学附属第一医院口腔科，重庆市 400016 doctor-mt@hotmail.com
  • 作者简介:郑钧元★，男，1984年生，上海市人，汉族，重庆医科大学在读硕士，主要从事牙周组织再生材料与牙周治疗药物方面的研究。 weiweig5yy@163.com

Biocompatibility of electro-spinning beta-tricalcium phosphate/gelatin guided tissue regeneration membrane versus polylactic acid and poly(lactide-co-glycolide) membrane

Zheng Jun-yuan1, Tang Ming1, Deng Xu-liang2, Yang Xiao-ping3, Zhang Shen3, Li Xiao-xia1   

  1. 1 Department of Stomatology, First Affiliated Hospital, Chongqing Medical University, Chongqing  400016, China; 2 School of Stomatology, Peking University, Beijing 100081, China, 3 Composites Center, Beijing University of Chemical Technology, Beijing 100029, China
  • Online:2010-04-16 Published:2010-04-16
  • Contact: Tang Ming, Master, Associate professor, Department of Stomatology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China doctor-mt@hotmail.com
  • About author:Zheng Jun-yuan★ Studying for master’s degree, Department of Stomatology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China weiweig5yy@163.com

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摘要：

背景：以往有很多学者通过发泡法将β-磷酸三钙与明胶复合得到引导组织再生材料，但将其采用静电纺丝技术制成纤维膜的报道很少。
目的：制备新型引导组织再生膜，并比较其与聚乳酸，聚丙交酯/乙交酯膜的细胞毒性。
方法：采用静电纺丝法制备β-磷酸三钙/明胶引导组织再生膜，通过扫描电子显微镜对纤维膜表面进行观察。通过MTT实验对电纺β-磷酸三钙/明胶膜与聚乳酸，聚丙交酯/乙交酯膜的细胞毒性进行对比。
结果与结论：新型电纺β-磷酸三钙/明胶膜为多孔状，β-磷酸三钙颗粒呈结节状附着在明胶纤维表面，纤维直径平均为400~500 nm，分布比较集中，大多在200~500 nm之间。与聚乳酸，聚丙交酯/乙交酯膜及阴性对照之间细胞毒性差异无显著性意义(P > 0.05)。说明新型电纺β-磷酸三钙/明胶引导组织再生膜细胞毒性较低，基本符合生物材料安全性的要求，有望成为新型的引导组织再生膜材料之一。

关键词: 明胶, &beta, -磷酸三钙, 聚乳酸, 聚丙交酯/乙交酯, 引导组织再生, 静电纺丝, 生物相容性

Abstract:

BACKGROUND: In the past, guided tissue regeneration materials were produced by β-tricalcium phosphate (β-TCP) and gelatin (Gel), but the production of fiber membrane by electro-spinning technique was reported less.
OBJECTIVE: To prepare a new kind of Gel with β-TCP hybrid nanofibrous membrane and testing its biocompatibility compared with polylactic acid (PLLA) and poly(lactide-co-glycolide) (PLGA) membrane.
METHODS: The β-TCP/Gel guided tissue regeneration membrane was made by electro-spinning technique. Scanning electron microscopy (SEM) was employed to observe membrane surface. MTT test was performed to compare toxicity among β-TCP/Gel, PLLA, and PLGA membranes.
RESULTS AND CONCLUSION: The β-TCP/Gel membrane was porous, and β-TCP granules were nodosity-adhered on surface of Gel. The average diameter of fiber was 500-600 nm. The distribution of fiber ranged from 200 to 500 nm. There was no significant difference in toxicity among PLLA, PLGA, and negative control group (P > 0.05). The results suggested that the β-TCP /Gel membrane was low cytotoxicity and suitable for tissue engineering. It would be a promising material for periodontal tissue regeneration.

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