中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (16): 2870-2874.doi: 10.3969/j.issn.1673-8225.2010.16.006

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

三种生物材料修复兔关节软骨缺损的性能对比

郭忠鹏，彭  超，蒋电明，安  洪   

  1. 重庆医科大学附属第一医院骨科，重庆市 400016
  • 出版日期:2010-04-16 发布日期:2010-04-16
  • 通讯作者: 蒋电明，主任医师，博士生导师，教授，重庆医科大学附属第一医院骨科，重庆市 400016 jdm571026@vip.163.com
  • 作者简介:郭忠鹏★，男，1980年生，山西省吕梁市人，汉族，在读硕士，医师，主要从事关节外科的研究。 dipeng_2001@163.com
  • 基金资助:

    课题为国家(973)纳米研究计划合作项目(2007CB936102)。

Performance comparison among three biomaterials in repairing articular cartilage defects

Guo Zhong-peng, Peng Chao, Jiang Dian-ming, An Hong   

  1. Department of Orthopaedics, First Affiliated Hospital, Chongqing Medical University, Chongqing  400016, China
  • Online:2010-04-16 Published:2010-04-16
  • Contact: Jiang Dian-ming, Chief physician, Doctoral supervisor, Professor, Department of Orthopaedics, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China jdm571026@vip.163.com
  • About author: Guo Zhong-peng★, Studying for master’s degree, Physician, Department of Orthopaedics, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China dipeng_2001@163.com
  • Supported by:

    the National Nanometer Research Cooperation Program (973 Program) No. 2007CB936102*

PDF

501

被引次数

17

摘要：

背景：聚乙烯醇水凝胶目前普遍被认为是一种在临床上非常有前途的关节软骨替代材料。聚酰胺66是一种具有高强度、高韧性和良好稳定性的聚合物，在工程和医学领域已获得广泛应用。聚氨酯具有良好的组织相容性和血液相容性，在合成材料中，聚氨酯在软骨修复方面表现出其独特的优势。
目的：比较多孔网状聚乙烯醇/聚氨酯/聚酰胺66生物材料修复兔关节软骨缺损的效果，探索性能更加优异的软骨支架材料。
方法：在36只兔髌股关节面上制造软骨缺损模型后按随机数字表法分为4组，每组9只。聚乙烯醇植入组、聚氨酯植入组、聚酰胺66植入组分别在软骨缺损处相应植入生物材料，对照组不做任何处理。植入4，8，12周后观察兔行为形态及膝关节局部组织形态学变化，对植入后12周修复组织进行组织学评分。
结果与结论：生物材料植入后兔大体形态观察伤口无感染，关节活动度好。植入后12周，植入组材料与周边组织融为一体，周边软骨未见明显退行性变；对照组整个观察期内，修复区表面不平整，与周围软骨分界清楚，有纤维状物覆盖。组织学评分显示聚乙烯醇植入组与聚氨酯植入组差异无显著性意义(P > 0.05)，优于聚酰胺66植入组及对照组(P < 0.05)。提示多孔聚乙烯醇/聚氨酯生物材料具有良好的组织相容性及替代关节软骨的功能。

关键词: 聚乙烯醇, 聚氨酯, 聚酰胺, 生物支架材料, 关节软骨, 缺损, 修复

Abstract:

BACKGROUND: Polyvinyl alcohol hydrogel (PVA) has been generally considered as a good substitute material of articular cartilage. Polyamide 66 (PA66) polymer has achieved an extensive application in the fields of engineering and medicine owing to its high intensity, strong tenacity and good stability. Polyurethanes exhibit good histocompatibility and blood compatibility and show distinct advantages in repairing cartilage. 
OBJECTIVE: To compare the outcomes of repairing articular cartilage defects in rabbit knee joints with three materials and to find the best material.
METHODS: Thirty-six healthy adult New Zealand rabbits were prepared for the models of articular cartilage defects by perforating on the patellofemoral joint and randomly divided into 4 groups, with 9 animals in each group. PVA, polyurethane, and PA66 were implanted into the into articular cartilage defects in rabbits of corresponding groups. There was no treatment in the control group. The macroscopic and histological evaluations of rabbit knee joints were performed at 4, 8 and 12 weeks postoperatively. The score of histology was used to evaluate the repairs of articular cartilage defects at 12 weeks postoperatively.
RESULTS AND CONCLUSION: No infection was observed in all animals with good motion of joints. At 12 weeks postoperatively, the materials got well with surrounding cartilage, which did not degenerate obviously. The defects in the control group were filled with fibrous scar-like tissues that arranged in disorder. The histological score of the in the group implanted by PVA, as well as polyurethane, was higher than that in PA66 and the control groups (P < 0.05), but the difference between the two groups had no significance (P > 0.05). The porous materials PVA and polyurethane have good function for replacing articular cartilage, with good biocompatibility.

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