中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (15): 2667-2670.doi: 10.3969/j.issn.1673-8225.2010.15.002

• 骨组织构建 bone tissue construction • 上一篇    下一篇

被动应力刺激兔冈上肌腱急性断裂修复后腱-骨界面的组织学变化

李  森，靳安民，付国建，王鹏成，张  辉，闵少雄   

  1. 南方医科大学珠江医院骨科中心，广东省广州市   510282
  • 出版日期:2010-04-09 发布日期:2010-04-09
  • 通讯作者: 靳安民，博士，教授，主任医师，博士后导师，南方医科大学珠江医院骨科中心，广东省广州市 510282 jinanmin2008@163.com
  • 作者简介:李 森☆，男，1979年生，河南省西华县人，汉族，南方医科大学在读博士，主治医师，主要从事脊柱、脊髓的损伤与防治的研究。 jht187@163.com

Effect of continuous passive motion on histological changes of tendon-bone interface following acute rupture supraspinatus tendon repair in rabbits  

Li Sen, Jin An-min, Fu Guo-jian, Wang Peng-cheng, Zhang Hui, Min Shao-xiong   

  1. Orthopedics Center of Zhujiang Hospital, Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Online:2010-04-09 Published:2010-04-09
  • Contact: Jin An-min, Doctor, Chief physician, Professor, Postdoctoral supervisor, Orthopedics Center of Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China jinanmin2008@163.com
  • About author:Li Sen☆, Studying for doctorate, Attending physician, Orthopedics Center of Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China jht187@163.com

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被引次数

1

摘要：

背景：肩袖损伤治疗的方法很多，但是对其损伤修复机制仍不是非常清楚，尤其是对腱-骨特殊结构的损伤修复机制研究更少。
目的：通过建立兔冈上肌腱急性断裂术后被动应力刺激(CPM)训练实验动物模型，观察被动应力刺激对其腱-骨界面组织学变化的影响，探讨被动应力刺激在肩袖损伤术后康复中的作用。
方法：选取20只成年雄性新西兰白兔，随机选取2只处死作正常对照，余18只白兔建立双侧肩关节行冈上肌腱急性断裂重建模型。1周后拆除石膏，随机选取2只处死作模型对照。余16只白兔随机分为被动应力刺激组8只与非被动应力刺激组8只。被动应力刺激组建模后第2周开始训练；非被动应力刺激组正常笼养。分别于术后第2，4，6，8周训练结束每组分别各处死2只，取材行腱-骨界面大体及组织学观察。
结果与结论：建模后2周，与非被动应力刺激组比较，被动应力刺激组炎症反应较重，术后4周，被动应力刺激组炎症较前明显减轻，术后第6周，被动应力刺激组组织结构学显示成纤维细胞数量较非被动应力刺激组增多，且逐渐有序，术后8周，显示被动应力刺激组成纤维细胞较非被动应力刺激组明显增多，且形状及排列较后者更加有序。结果证实采用被动应力刺激(CPM)对兔冈上肌腱急性断裂重建后进行训练，能够促进其腱-骨修复，从而促进肩袖损伤功能恢复。

关键词: 冈上肌腱, 腱-骨修复, 被动应力刺激, 兔, 组织构建, 肌肉肌腱组织工程

Abstract:

BACKGROUND: Rotator cuff injuries can be treated by many methods, but their mechanisms of repairing are still unclear, especially for tendon-bone injury.
OBJECTIVE: To establish continuous passive motion (CPM) models following repairing acute rupture of supraspinatus tendon, to observe the effect of CPM on histological changes of tendon-bone, and to explore the role of CPM in the postoperative rehabilitation following acute rotator cuff injury .
METHODS: Twenty adult male New Zealand white rabbits were selected, 2 of them were randomly sacrificed as controls, and the remained animals were established rabbit rotator cuff injury prosthetic experimental models. Two rabbits were sacrificed as model controls, and others were divided into the CPM (n=8) and non-CPM (n=8) groups. CPM was performed on rabbits in the CPM group at 2 weeks after model preparation. Rabbits in the non-CPM were housed regularly. Two rabbits were sacrificed at weeks 2, 4, 6 and 8 after training, and the gross and histological observation of tendon-bone interface was performed. 
RESULTS AND CONCLUSION: Compared with the non-CPM group, the CPM group presented serious inflammatory response at 2 weeks, which was obviously decreased at 4 weeks. After 6 weeks, the number of fibroblasts of the CPM group was increased compared with the non-CPM group. After 8 weeks, the organizational structure study showed that fibroblasts in the CPM were greater than that of the non-CPM group, with orderly shape and arrangements. The results demonstrated that CPM can contribute to rabbit supraspinatus tendon-bone repair, so as to promote functional recovery.

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