中国组织工程研究

中国组织工程研究 ›› 2025, Vol. 29 ›› Issue (8): 1634-1642.doi: 10.12307/2025.336

• 肌肉肌腱韧带组织构建 tissue construction of the muscle, tendon and ligament • 上一篇    下一篇

低频脉冲磁场慢性暴露对健康成年股四头肌收缩力及形态的影响

王选强1，张文洋2，李  阳3，孔维签4，李  伟4，王  乐1，厉中山5，6，白  石4   

  1. 1大连海洋大学体育部，辽宁省大连市  116023；东北大学，2信息科学与工程学院，5体育部，辽宁省沈阳市  110819；3大连医科大学附属第二医院超声科，辽宁省大连市  116021；4沈阳工业大学信息科学与工程学院，辽宁省沈阳市  110034；6福建师范大学体育科学学院，福建省福州市  350117
  • 收稿日期:2024-03-04 接受日期:2024-04-02 出版日期:2025-03-18 发布日期:2024-07-05
  • 通讯作者: 王乐，博士，教授(三级)，高校体育教学与管理，大连海洋大学体育部，辽宁省大连市 116023 并列通讯作者：厉中山，福建师范大学体育科学学院在读博士，东北大学体育部教师，辽宁省沈阳市 110819；福建师范大学体育科学学院，福建省福州市 350117 并列通讯作者：白石，博士，博士生导师，“翔源学者”特聘教授，沈阳工业大学信息科学与工程学院，辽宁省沈阳市 110034
  • 作者简介:王选强，男，1969年生，汉族，副教授，主要从事高校体育教学与运动训练学研究。
  • 基金资助:
    国家自然科学基金(62001313)，项目负责人：白石；省教育科学“十四五”规划项目，项目负责人：王乐；辽宁省科技重大重点项目(2021JH2/10300134，2022JH1/10500004)，项目负责人：白石；辽宁省自然科学基金(2022-YGJC-11，2022-YGJC-29，2022-YGJC-35)，项目负责人：白石；辽宁省人才项目(XLYC2203046)，项目负责人：白石；辽宁省教育厅重点项目(LJKZ0133)，项目负责人：白石；沈阳市重大项目(22-321-32-09)，项目负责人：白石；中国大学生体育协会一般项目(L202103003)，项目负责人：厉中山

Effects of chronic exposure to low-frequency pulsed magnetic fields on contractility and morphology of the quadriceps muscle in healthy adults

Wang Xuanqiang1, Zhang Wenyang2, Li Yang3, Kong Weiqian4, Li Wei4, Wang Le1, Li Zhongshan5, 6, Bai Shi4   

  1. 1Department of Physical Education, Dalian Ocean University, Dalian 116023, Liaoning Province, China; 2College of Information Science and Engineering, 5Department of Physical Education, Northeastern University, Shenyang 110819, Liaoning Province, China; 3Department of Ultrasound, The Second Affiliated Hospital of Dalian Medical University, Dalian 116021, Liaoning Province, China; 4School of Information Science and Engineering, Shenyang University of Technology, Shenyang 110034, Liaoning Province, China; 6School of Physical Education and Sports Science, Fujian Normal University, Fuzhou 350117, Fujian Province, China
  • Received:2024-03-04 Accepted:2024-04-02 Online:2025-03-18 Published:2024-07-05
  • Contact: Wang Le, Department of Physical Education, Dalian Ocean University, Dalian 116023, Liaoning Province, China Co-corresponding author: Li Zhongshan, MD candidate, Department of Physical Education, Northeastern University, Shenyang 110819, Liaoning Province, China; School of Physical Education and Sports Science, Fujian Normal University, Fuzhou 350117, Fujian Province, China Co-corresponding author: Bai Shi, PhD, Doctoral supervisor, School of Information Science and Engineering, Shenyang University of Technology, Shenyang 110034, Liaoning Province, China
  • About author:Wang Xuanqiang, Associate professor, Department of Physical Education, Dalian Ocean University, Dalian 116023, Liaoning Province, China
  • Supported by:
    the National Natural Science Foundation of China, No. 62001313 (to BS); the 14th Five-Year Plan of Liaoning Provincial Education Science Program (to WL); Liaoning Provincial Major Science and Technology Project, Nos. 2021JH2/10300134 and 2022JH1/10500004 (both to BS); Liaoning Provincial Natural Science Foundation, Nos. 2022-YGJC-11, 2022-YGJC-29, and 2022-YGJC-35 (all to BS); Liaoning Provincial Talent Project, No. XLYC2203046 (to BS); Liaoning Provincial Department of Education Key Project, No. LJKZ0133 (to BS); Shenyang Municipal Major Project, No. 22-321-32-09 (to BS); General Project of Federation of University Sports of China, No. L202103003 (to LZS)

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摘要：


文题释义：
脉冲磁场：由脉冲发生装备所产生的脉冲电流在线圈中产生的一种瞬态电磁场。
经典瞬时受体电位通道：是一类主要透Ca2+的非选择性阳离子通道，参与细胞内Ca2+的调节，在细胞的生理功能的调节中发挥了重要的作用。

背景：目前已有关于低频脉冲磁场对骨骼肌结构形态影响研究发现了骨骼肌质量变化，但对接受慢性暴露后的形态变化未见相关研究。
目的：观察低频脉冲磁场慢性暴露对腿部股四头肌最大自主收缩力与形态指标的影响，为使用该技术作为肌肉功能改善策略提供组织形态变化的参考依据。
方法：招募普通健康受试者70名，随机分为接受磁场刺激的试验组与进行假治疗的对照组，每组35名，试验时长共计4周，试验组受试者每隔48 h进行1次15 min的低频脉冲磁刺激干预，对照组进行假治疗，干预间隔与时长与试验组一致，干预4周后观察不同组别受试者股四头肌最大自主收缩力值变化，利用B型超声影像作为评估手段，观察肌肉厚度、肌肉横截面积及羽状角指标的变化。
结果与结论：①经过4周的低频脉冲磁场慢性暴露，68名受试者完成试验，试验组受试者股四头肌最大自主收缩力测试值显著增长(P=0.000)，提升幅度显著高于对照组(P=0.008)；②试验组受试者的肌肉形态3项指标均显著高于试验前测(P=0.000)，而对照组肌肉厚度指标呈显著性下降(P=0.020)，羽状角无显著性变化，横截面积显著性提升(P=0.000)；③组间对比发现，试验组关于肌肉形态的肌肉厚度(P=0.012)、羽状角(P=0.003)及横截面积(P=0.049)3项指标均显著高于对照组；④上述数据证实，健康成年受试者经过4周的低频脉冲磁场慢性暴露，股四头肌的最大自主收缩力显著提升，同时观察到试验组的肌肉厚度、横截面积及羽状角3项肌肉形态指标的显著增长，对使用该技术作为肌肉改善的运动替代与医疗策略提供了肌组织形态改善的支持依据。
https://orcid.org/0009-0008-0488-5324(王选强)；https://orcid.org/0000-0002-9010-609X(厉中山)；https://orcid.org/0000-0001-5297-6353(白石)

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 低频脉冲磁场, 慢性暴露, 瞬时感受器电位通道1, TRPC1, 骨骼肌形态结构, 最大自主收缩力, 肌骨超声, 肌肉厚度, 肌肉横截面积, 羽状角, 双盲试验

Abstract: BACKGROUND: Changes in skeletal muscle mass have been indicated in studies addressing the effects of low-frequency pulsed magnetic fields on the structure and morphology of the skeletal muscle, but no relevant studies have been conducted on the morphologic changes that occur after chronic exposure to the low-frequency pulsed magnetic field.
OBJECTIVE: To observe the effects of chronic exposure to low-frequency pulsed magnetic fields on the maximal voluntary contraction and morphologic indicators of the quadriceps muscle of the leg, thereby providing a reference of muscle morphologic changes for the use of this technique as a strategy for muscle function improvement.
METHODS: Seventy healthy subjects were recruited and randomly divided into a test group that received magnetic field stimulation and a control group that underwent sham treatment, with 35 subjects in each group, and the total duration of the trial was 4 weeks. The test group underwent low-frequency pulsed magnetic stimulation for 15 minutes every 48 hours, while the control group underwent sham treatment, with the same intervention interval and duration as the test group. After 4 weeks of intervention, changes in the maximum voluntary contraction value of the quadriceps muscle in different groups were observed, and B-mode ultrasonography was utilized as a means of assessment to observe changes in muscle thickness, muscle cross-sectional area, and pinnation angle indexes.
RESULTS AND CONCLUSION: After 4 weeks of chronic exposure to low-frequency pulsed magnetic fields, 68 subjects completed the test. The maximum voluntary contraction value of the quadriceps muscle in the test group increased significantly (P=0.000), and the increment was significantly higher than that of the control group (P=0.008). Three indexes related to muscle morphology in the test group were significantly higher than the pre-test values (P=0.000), while in the control group, muscle thickness showed a significant reduction (P=0.020), there was no significant change in the pinnation angle, but a significant increase in the cross-sectional area (P=0.000). Intergroup comparisons revealed that the three indicators related to muscle morphology, including muscle thickness (P=0.012), pinnation angle (P=0.003), and cross-sectional area (P=0.049), were significantly higher in the test group than in the control group. The above data confirmed that the maximum voluntary contraction of the quadriceps muscle was significantly increased in healthy adults after 4 weeks of chronic exposure to the low-frequency pulsed magnetic field, and significant increases in the three muscle morphometric indices of muscle thickness, cross-sectional area, and pinnation angle were observed in the test group, providing a basis of muscle tissue morphology for the use of this technique as an exercise alternative and medical treatment strategy for muscle improvement.

Key words: low-frequency pulsed magnetic field, chronic exposure, transient receptor potential channel 1, TRPC1, skeletal muscle morphologic structure, maximal voluntary contraction, musculoskeletal ultrasound, muscle thickness, muscle cross-sectional area, pinnation angle, double-blind trial

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