中国组织工程研究

中国组织工程研究 ›› 2025, Vol. 29 ›› Issue (2): 402-408.doi: 10.12307/2025.231

• 神经组织构建 nerve tissue construction • 上一篇    下一篇

电针联合低频经颅超声刺激对创伤性脑损伤大鼠脑电信号的影响

高思淼1，2，韩  雪3，吴晓光1，郑金钰2，高芳雯2，李葵花1，2，4，彭  勇5，6，刘兰祥7   

  1. 承德医学院，1河北省神经损伤与修复重点实验室，2生物医学工程系，4河北省医工结合国际研究中心，河北省承德市  067000；3承德医学院附属医院神经内科，河北省承德市  067000；燕山大学，5电气工程学院，6河北省智能康复及神经调控重点实验室，河北省秦皇岛市  066004；7秦皇岛市第一医院，河北省秦皇岛市  066004

  • 收稿日期:2023-12-08 接受日期:2024-02-04 出版日期:2025-01-18 发布日期:2024-05-25
  • 通讯作者: 李葵花，硕士，副教授，硕士生导师，承德医学院，河北省神经损伤与修复重点实验室，生物医学工程系，河北省医工结合国际研究中心，河北省承德市 067000
  • 作者简介:高思淼，女，1997年生，北京市人，承德医学院在读硕士，主要从事神经损伤修复研究。
  • 基金资助:
    河北省神经损伤与修复重点实验室开放课题(NJKF202305)，项目负责人：李葵花；河北省卫健委医学科学研究课题(20231558)，项目负责人：吴晓光；国家自然科学基金(81871029)，项目负责人：刘兰祥

Effect of electroacupuncture combined with low-frequency transcranial ultrasound stimulation on the electroencephalographic signals of rats with traumatic brain injury 

Gao Simiao1, 2, Han Xue3, Wu Xiaoguang1, Zheng Jinyu2, Gao Fangwen2, Li Kuihua1, 2, 4, Peng Yong5, 6, Liu Lanxiang7   

  1. 1Key Laboratory of Nerve Injury and Repair of Hebei Province, 2Department of Biomedical Engineering, 4International Research Center for Medical-Industrial Integration of Hebei, Chengde Medical University, Chengde 067000, Hebei Province, China; 3Department of Neurology, Affiliated Hospital of Chengde Medical University, Chengde 067000, Hebei Province, China;  5Institute of Electrical Engineering, 6Key Laboratory of Intelligent Rehabilitation and Neuromodulation of Hebei Province, Yanshan University, Qinhuangdao 066004, Hebei Province, China; 7First Hospital of Qinhuangdao, Qinhuangdao 066004, Hebei Province, China

  • Received:2023-12-08 Accepted:2024-02-04 Online:2025-01-18 Published:2024-05-25
  • Contact: Li Kuihua, Master, Associate professor, Master’s supervisor, Key Laboratory of Nerve Injury and Repair of Hebei Province, Chengde Medical University, Chengde 067000, Hebei Province, China; Department of Biomedical Engineering, Chengde Medical University, Chengde 067000, Hebei Province, China; International Research Center for Medical-Industrial Integration of Hebei, Chengde Medical University, Chengde 067000, Hebei Province, China
  • About author:Gao Simiao, Master candidate, Key Laboratory of Nerve Injury and Repair of Hebei Province, Chengde Medical University, Chengde 067000, Hebei Province, China; Department of Biomedical Engineering, Chengde Medical University, Chengde 067000, Hebei Province, China
  • Supported by:
    Open Project of Hebei Provincial Key Laboratory of Nerve Injury and Repair, No. NJKF202305 (to LKH); Medical Science Research Project of Hebei Provincial Health Commission, No. 20231558 (to WXG); National Natural Science Foundation of China, No. 81871029 (to LLX)

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摘要：

文题释义：
低频经颅超声刺激：指在无创条件下，将低频、低功率的超声信号透过颅骨传导至大脑，从而调控神经活动。
脑电信号处理：脑电信号来源于大脑皮质神经元的波动活动，神经元的树突上发生突触兴奋时产生电流。这些神经放电现象被信号采集器采集后经放大、处理并存储于计算机中，用计算机对信号进行分析，分解出不同脑电频段，并计算脑电各项指标，这些指标的变化可反映脑组织损伤异常情况。

背景：创伤性脑损伤是由头部受到撞击、打击而导致大脑正常功能被破坏的疾病，目前需要寻找有效治疗方式和客观指标，帮助医生判别损伤状况及恢复患者脑功能。
目的：探究电针联合低频经颅超声刺激对创伤性脑损伤大鼠脑电信号的影响。
方法：将40只6周龄SPF级雄性SD大鼠随机分为假手术组、模型组、电针组、低频经颅超声刺激组和联合组(n=8)，后4组采用Feeney自由落体法造模，假手术组只开骨窗而不打击。各干预组均于造模后1 d开始实施干预，电针组进行电针干预，低频经颅超声刺激组进行低频经颅超声刺激干预，联合组进行两者联合干预，共干预7 d。造模后8 h，用改良神经功能缺损评分评定大鼠神经功能缺损情况；干预7 d后观察大鼠Y迷宫自发轮流行为百分比，而后采集脑电信号，利用快速傅里叶变换分解出α、β、θ和δ波段，计算各频段振荡幅值、能量占比百分比以及Lempel-Ziv复杂度、样本熵。
结果与结论：①造模后8 h，模型组、电针组、低频经颅超声刺激组和联合组的改良神经功能缺损评分显著高于假手术组(P < 0.05)；②造模后第7天，模型组的α波、δ波频带振荡幅值、δ波能量占比百分比显著高于假手术组(P < 0.05)，自发轮流行为百分比、α波、β波能量占比百分比、Lempel-Ziv复杂度、样本熵显著低于假手术组(P < 0.05)；③与模型组比较，联合组的α波、δ波频带振荡幅值显著下降(P < 0.05)，电针组、低频经颅超声刺激组、联合组的α波、β波频带能量占比百分比显著升高(P < 0.05)，δ波能量占比百分比显著下降(P < 0.05)；④与电针组和低频经颅超声刺激组相比，联合组的δ波能量占比百分比显著降低(P < 0.05)，自发轮流行为百分比、α波、β波能量占比百分比、Lempel-Zi复杂度、样本熵显著升高(P < 0.05)；⑤结果显示，创伤性脑损伤大鼠出现脑电信号异常，而电针联合低频经颅超声刺激干预后可以改善大鼠脑电信号的异常情况，提示脑电频域特征和非线性特征可用来评估创伤性脑损伤情况。 

关键词: 创伤性脑损伤, 电针, 低频经颅超声刺激, 脑电信号, 神经功能

Abstract: BACKGROUND: Traumatic brain injury is a condition in which the normal function of the brain is disrupted by a bump or impact to the head. It is necessary to find effective treatments and objective targets that can help doctors diagnose the injury status and restore the brain function of patients.
OBJECTIVE: To explore the effect of electroacupuncture combined with low-frequency transcranial ultrasound stimulation on the electroencephalographic signals of rats with traumatic brain injury.
METHODS: Forty 6-week-old SPF male Sprague-Dawley rats were randomly divided into five groups: sham group, model group, electroacupuncture group, low-frequency transcranial ultrasound stimulation group and combined group (electroacupuncture+low-frequency transcranial ultrasound stimulation), with eight rats in each group. Feeney weight-drop method was used to establish the animal model of traumatic brain injury. In the sham group, the bone window was only opened without impact. Interventions were started at 1 day after modeling. Electroacupuncture in the electroacupuncture group, low-frequency transcranial ultrasound stimulation in the low-frequency transcranial ultrasound stimulation group, and electroacupuncture+low-frequency transcranial ultrasound stimulation in the combined group were performed for days in total. The modified neurological severity scale score for assessing rats’ neurological deficits was performed at 8 hours after modeling. The percentage of spontaneous alternation behavior in the Y-maze was measured at 7 days after modeling. Then, the electroencephalographic signals were collected and electroencephalographic data of α, β, θ, and δ waves were extracted by fast Fourier transform, and the value of oscillation amplitude and energy ratio were calculated in α, β, θ, and δ waves, as well as the Lempel-Ziv complexity and sample entropy.
RESULTS AND CONCLUSION: Compared with the sham group, the modified neurological severity scale scores in the model group, electroacupuncture group, low-frequency transcranial ultrasound stimulation group and combined group were significantly increased at 8 hours after modeling (P < 0.05). Compared with the sham group, the value of oscillation amplitude in δ wave and the value of δ energy ratio were significantly increased in the model group at 7 days after modeling, meanwhile the percentage of spontaneous alternation behavior in Y-maze, and the value of α/β energy ratio, Lempel-Ziv complexity, and sample entropy were significantly decreased (P < 0.05). Compared with the model group, the value of oscillation amplitude in α and δ waves was significantly decreased in the combined group (P < 0.05), while the value of α/β energy ratio was significantly increased (P < 0.05) and the value of δ energy ratio was significantly decreased (P < 0.05) in the electroacupuncture group, low-frequency transcranial ultrasound stimulation group and combined group. Compared with the electroacupuncture group and low-frequency transcranial ultrasound stimulation group, the value of δ energy ratio was significantly decreased in the combined group (P < 0.05), while the percentage of spontaneous alternation behavior, the value of α/β energy ratio, the Lempel-Ziv complexity, and the sample entropy were significantly increased (P < 0.05). To conclude, abnormal electroencephalographic signals can appear in rats with traumatic brain injury, while the electroacupuncture combined with low-frequency transcranial ultrasound stimulation can alleviate the abnormal electroencephalographic signals in rats, which suggests the electroencephalographic frequency domain value and nonlinear features can be used to assess the severity of traumatic brain injury.


Key words: traumatic brain injury, electroacupuncture, low-frequency transcranial ultrasound stimulation, electroencephalographic signal, neurological function

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