中国组织工程研究

中国组织工程研究 ›› 2024, Vol. 28 ›› Issue (32): 5141-5147.doi: 10.12307/2024.498

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

高强度间歇训练影响绝经后骨质疏松模型大鼠骨健康的机制

杨  瑞1，曹  凯2，赵  伟2，王庆博3，陆春敏3，张  艳3   

  1. 1中国矿业大学体育学院，江苏省徐州市  221116；2中国人民武装警察部队后勤学院，天津市  300309；3广西中医药大学体育部，广西壮族自治区南宁市  530021
  • 收稿日期:2023-08-31 接受日期:2023-09-25 出版日期:2024-11-18 发布日期:2023-12-28
  • 通讯作者: 张艳，硕士，副教授，广西中医药大学体育部，广西壮族自治区南宁市 530021
  • 作者简介:杨瑞，女，1996年生，山东省枣庄市人，汉族，硕士，助教，主要从事运动与健康研究。
  • 基金资助:
    广西教育科学“十三五”规划课题(2017C386)，项目负责人：张艳

Mechanisms by which high-intensity interval training influences bone health in a rat model of postmenopausal osteoporosis

Yang Rui1, Cao Kai2, Zhao Wei2, Wang Qingbo3, Lu Chunmin3, Zhang Yan3   

  1. 1School of Physical Education, China University of Mining and Technology, Xuzhou 221116, Jiangsu Province, China; 2Logistics University of Chinese People’s Armed Police Forces, Tianjin 300309, China; 3Department of Physical Education, Guangxi University of Chinese Medicine, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Received:2023-08-31 Accepted:2023-09-25 Online:2024-11-18 Published:2023-12-28
  • Contact: Zhang Yan, Master, Associate professor, Department of Physical Education, Guangxi University of Chinese Medicine, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • About author:Yang Rui, Master, Assistant researcher, School of Physical Education, China University of Mining and Technology, Xuzhou 221116, Jiangsu Province, China
  • Supported by:
    Guangxi Education Science "13th Five-Year Plan" Project, No. 2017C386 (to ZY)

PDF

89

摘要：


文题释义：

高强度间歇训练：是近年来竞技体育、大众健身与康复医学界新兴的一种省时高效运动模式，以超过无氧阈或最大乳酸稳态负荷强度进行多次持续时间为几秒到几分钟的练习，且每两次练习之间安排使练习者不足以完全恢复静息或低强度练习的训练方法。
肌肉因子：是在运动中由骨骼肌细胞响应肌肉收缩分泌和释放的细胞因子或肽类物质，可通过自分泌、旁分泌、内分泌发挥作用，即肌肉因子除能够调节自身代谢和邻近器官外，还可随血液循环作用于全身多个脏器(如脂肪、肝脏、大脑、肌肉、骨骼等)，参与机体代谢等生理进程。


背景：改善骨质疏松患者骨健康推荐的运动方式有抗阻运动和负重训练，高强度间歇训练属于高冲击性负重运动且具有明显时效性特征，然而其对骨骼的影响鲜有报道。

目的：观察高强度间歇训练对去卵巢模型大鼠骨健康的影响。
方法：采用随机数字表法将36只雌性SD大鼠分为假手术组、模型组和模型运动组，每组12只。切除模型组和模型运动组大鼠双侧卵巢建立骨质疏松模型，造模6周后，模型运动组大鼠进行高强度间歇训练(以90%峰值跑速运动2 min、50%峰值跑速运动1 min为1个循环，共完成9个循环，3 d/周)，假手术组和模型组大鼠同期在鼠笼内安静饲养，共训练6周。末次训练后48-72 h取材进行相关指标检测。

结果与结论：①与假手术组比较，模型组大鼠胫骨骨密度、最大载荷、刚度、弹性、骨小梁体积分数、骨小梁数量降低(P < 0.05)，骨小梁分离度升高(P < 0.05)；血清、腓肠肌和股骨内鸢尾素含量降低(P < 0.05)；腓肠肌过氧化物酶体增殖体激活受体γ共激活因子1α蛋白表达以及Ⅲ型纤连蛋白结构域蛋白5 mRNA和蛋白表达下降(P < 0.05)；股骨Ⅰ型胶原、Osterix、Runx2 mRNA表达下降(P < 0.05)，抗酒石酸酸性磷酸酶、核因子κB受体活化因子配体、破骨细胞关联受体mRNA表达升高(P < 0.05)。②与模型组比较，模型运动组大鼠胫骨骨密度、断裂载荷、最大载荷、刚度、弹性、骨小梁平均厚度、骨小梁数量升高(P < 0.05)，骨小梁分离度降低(P < 0.05)；血清、腓肠肌和股骨内鸢尾素含量升高(P < 0.05)；腓肠肌过氧化物酶体增殖体激活受体γ共激活因子1α蛋白表达以及Ⅲ型纤连蛋白结构域蛋白5 mRNA和蛋白表达升高(P < 0.05)；股骨Ⅰ型胶原、Osterix、Runx2 mRNA表达升高(P < 0.05)，抗酒石酸酸性磷酸酶、核因子κB受体活化因子配体、破骨细胞关联受体mRNA表达下降(P < 0.05)。③结果表明，短期高强度间歇训练可能通过上调骨骼肌肌肉因子鸢尾素水平改善去卵巢大鼠骨健康水平。

https://orcid.org/0009-0001-5959-6916(杨瑞)

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 高强度间歇训练, 绝经后骨质疏松, 骨骼肌, 鸢尾素, 骨健康

Abstract: BACKGROUND: Resistance training and weight-bearing exercise are recommended modes for patients with osteoporosis to improve bone health. High-intensity interval training is a high-impact weight-bearing exercise with obvious time-efficient characteristics; however, little attention has been paid to its impact on bones.
OBJECTIVE: To observe the effect of high-intensity interval training on the bone health of ovariectomized rat models.
METHODS: Thirty-six female Sprague-Dawley rats were randomly divided into sham group, model group and model exercise group (n=12 per group). Bilateral ovariectomy was used to prepare an osteoporosis rat model in the latter two groups. Six weeks after modeling, the model exercise group was subjected to a high-intensity interval training on an electric treadmill at 90% peak running speed for 2 minutes and 50% peak running speed for 1 minute as one session, a total of nine sessions, 3 days per week, for 6 weeks. Rats in the sham and model groups were raised quietly in the mouse cage during the same period. The relevant indexes were tested 48-72 hours after the final training.
RESULTS AND CONCLUSION: Compared with the sham group, bone mineral density, maximal load, stiffness, elasticity, trabecular volume fraction, and trabecular number decreased (P < 0.05), while trabecular separation increased (P < 0.05); the level of irisin in the serum, gastrocnemius and femur decreased (P < 0.05); the expression of peroxisome proliferator-activated receptor γ coactivator-1α protein and fibronectin type III domain-containing protein 5 mRNA and protein in the gastrocnemius muscle decreased (P < 0.05); the expression of type I collagen, Osterix, and Runx2 mRNA in the femur decreased (P < 0.05); and the expression of anti-tartrate acid phosphatase, receptor activator of nuclear factor κB ligand, and osteoclast-associated receptor mRNA increased in the model group (P < 0.05). Compared with the model group, bone mineral density, fracture load, maximal load, stiffness, elasticity, average trabecular thickness, and trabecular number increased (P < 0.05), and trabecular separation decreased (P < 0.05); the level of irisin in the serum, gastrocnemius and femur increased (P < 0.05); the expression of peroxisome proliferator-activated receptor γ coactivator-1α protein and fibronectin type III domain-containing protein 5 mRNA and protein in gastrocnemius increased (P < 0.05); the expression of type I collagen, Osterix, and Runx2 mRNA in the femur increased (P < 0.05); and the expression of anti-tartrate acid phosphatase, receptor activator of nuclear factor κB ligand, and osteoclast-associated receptor mRNA decreased in the model exercise group (P < 0.05). To conclude, short-term high-intensity interval training may improve bone health of ovariectomized rats through up-regulating the irisin level.

Key words: high-intensity interval training, postmenopausal osteoporosis, skeletal muscle, irisin, bone health

中图分类号: