中国组织工程研究

中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (26): 4113-4117.doi: 10.12307/2022.811

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

电针刺激对大鼠脊髓损伤部位神经胶质纤维酸性蛋白的影响

唐福宇1，周宾宾2，魏卫兵1，张鸿升3   

  1. 1柳州市中医医院(柳州市壮医医院)，广西壮族自治区柳州市   545000；2广西中医药大学第一附属医院，广西壮族自治区南宁市   543000；3广西中医药大学附属瑞康医院，广西壮族自治区南宁市   543000
  • 收稿日期:2021-05-27 接受日期:2021-07-05 出版日期:2022-09-18 发布日期:2022-03-07
  • 通讯作者: 周宾宾，主任医师，教授，广西中医药大学第一附属医院，广西壮族自治区南宁市 543000
  • 作者简介:唐福宇，男，1977年生，2011年广西中医院毕业，硕士，主任医师，主要从事中西医结合诊治脊柱脊髓相关疾病方面的研究。
  • 基金资助:
    国家自然科学基金(81660814)：电针通过Nogo-A/NgR与NGF/TrkA的crostalk调节SCI后神经可塑性的机制研究，项目负责人：周宾宾；广西自然科学基金(2019JJA140167)，项目负责人：唐福宇；柳州市科技计划项目(2019BE10602)：提针治疗背肌筋膜炎的临床研究及机制探讨，项目负责人：唐福宇

Effect of electroacupuncture on glial fibrillary acidic protein expression at the injured site in a rat model of spinal cord injury

Tang Fuyu1, Zhou Binbin2, Wei Weibing1, Zhang Hongsheng3   

  1. 1Liuzhou Traditional Chinese Medicine Hospital (Liuzhou Zhuang Medical Hospital), Liuzhou 545000, Guangxi Zhuang Autonomous Region, China; 2the First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 543000, Guangxi Zhuang Autonomous Region, China; 3Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 543000, Guangxi Zhuang Autonomous Region, China
  • Received:2021-05-27 Accepted:2021-07-05 Online:2022-09-18 Published:2022-03-07
  • Contact: Zhou Binbin, Chief physician, Professor, the First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 543000, Guangxi Zhuang Autonomous Region, China
  • About author:Tang Fuyu, Master, Chief physician, Liuzhou Traditional Chinese Medicine Hospital (Liuzhou Zhuang Medical Hospital), Liuzhou 545000, Guangxi Zhuang Autonomous Region, China
  • Supported by:
    the National Natural Science Foundation of China, No. 81660814 (to ZBB); the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 2019JJA140167 (to TFY); Science and Technology Project of Liuzhou City), No. 2019BE10602 (to TFY)

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摘要：

文题释义：
星形胶质细胞：脊髓损伤后星形胶质细胞会增生性地生长，使脊髓在结构形态上保持完整性，同时对神经组织也会起到一定的营养和支持作用。后期，星形胶质细胞持续被激活，分泌或合成抑制性的因子；同时胶质细胞过度增生，会在损伤区形成致密的胶质瘢痕。通过干细胞移植等方法能使轴突有效再生，但是再生的轴突接触到致密的胶质瘢痕时，其轴突的再生就会受到抑制，停止生长。
神经胶质纤维酸性蛋白：与星形胶质细胞的活化程度密切相关，是胶质瘢痕形成的主要标志。当神经胶质纤维酸性蛋白表达下降，胶质瘢痕的形成会受到明显抑制，轴突的芽生会明显增强，这不仅说明神经胶质纤维酸性蛋白对胶质瘢痕的重要性，同时也证实通过抑制神经胶质纤维酸性蛋白的表达能有效缓解胶质瘢痕的形成，对轴突的再生有很好的促进作用。

背景：神经胶质纤维酸性蛋白是一个中间丝过渡表达蛋白，其表达水平是最常用的星形胶质细胞的特异性标志蛋白。中医治疗脊髓损伤历史悠久，大量研究表明针刺“督脉”“足阳明胃经”对脊髓损伤的神经再生修复具有一定作用。
目的：观察电针刺激后脊髓损伤大鼠损伤部位神经胶质纤维酸性蛋白表达的变化，进一步探讨电针刺激对脊髓损伤大鼠神经再生的影响。
方法：将120只SD大鼠随机分为5组，即督脉电针组、胃经电针组、混合电针组、模型对照组和假手术组，每组24只，除假手术组不进行脊髓切断外，其他各组均制备T10脊髓半横断损伤模型。每个组再随机分为治疗后3，7，14，21 d组，每亚组6只。假手术组及模型对照组不进行干预，其他3个电针组进行相应电针干预。以免疫组化法检测损伤局部神经胶质纤维酸性蛋白阳性细胞的表达；PCR、Western blot检测神经胶质纤维酸性蛋白 mRNA及蛋白的表达情况。
结果与结论：①模型对照组损伤后神经胶质纤维酸性蛋白的表达水平呈先增高后降低的趋势，与未损伤的假手术组相比差异有显著性意义(P < 0.05)；②督脉电针组、胃经电针组与模型对照组相比，神经胶质纤维酸性蛋白表达水平明显降低(P < 0.05)；③混合电针组与督脉电针组、胃经电针组相比神经胶质纤维酸性蛋白表达水平降低，但差异无显著性意义(P > 0.05)；④提示电针刺激可降低损伤局部神经胶质纤维酸性蛋白的表达，减少损伤局部星形胶质细胞的表达，从而抑制胶质瘢痕的形成。
缩略语：胶质纤维酸性蛋白：glial fibrillary acidic protein，GFAP

https://orcid.org/0000-0002-4279-371X (唐福宇) 

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 电针刺激, 脊髓损伤, 胶质纤维酸性蛋白, 督脉, 胃经, 神经再生

Abstract: BACKGROUND: Glial fibrillary acidic protein is an intermediate filament overexpression protein, which is the most commonly used specific marker protein for astrocytes. Traditional Chinese medicine has a long history of treating spinal cord injury. Numerous studies have shown that acupuncture at Du Meridian and Stomach Meridian of Foot-Yangming has a certain therapeutic effect on nerve regeneration and repair after spinal cord injury.  
OBJECTIVE: To observe the changes in the expression of glial fibrillary acidic protein in the injured part of spinal cord injury rats after electroacupuncture stimulation, and to further investigate the effect of electroacupuncture on nerve regeneration in rats with spinal cord injury.
METHODS: A total of 120 Sprague-Dawley rats were randomly divided into 5 groups (n=24 per groups): a Du Meridian electroacupuncture group, a Stomach Meridian of Foot-Yangming electroacupuncture group, a mixed electroacupuncture group, a model control group, and a sham operation group. T10 spinal cord hemisection models were prepared in all the groups except for the sham operation group. After modeling, each group was randomly subdivided into four subgroups (n=6 per group): a 3-day group, a 7-day group, a 14-day group, and a 21-day group. The sham operation group and the model control group were not given an intervention, and the three electroacupuncture groups were given a corresponding electroacupuncture intervention. Immunohistochemical staining was used to detect the expression of glial fibrillary acidic protein positive cells in the injured area. PCR and western blot were used to detect the mRNA and protein expression of glial fibrillary acidic protein.  
RESULTS AND CONCLUSION: Compared with the sham operation group, the expression of glial fibrillary acidic protein after injury was increased first and then decreased significantly in the model control group (P < 0.05). Compared with the model control group, the expression of glial fibrillary acidic protein was significantly reduced in the Du Meridian electroacupuncture group and the Stomach Meridian of Foot-Yangming electroacupuncture group (P < 0.05). The expression of glial fibrillary acidic protein in the mixed electroacupuncture group was lower than that in the Du Meridian electroacupuncture group and the Stomach Meridian of Foot-Yangming electroacupuncture group, but there was no significant difference (P > 0.05). To conclude, electroacupuncture stimulation can reduce the expression of glial fibrillary acidic protein and astrocytes in the injured area, thereby inhibiting the formation of glial scars.

Key words: electroacupuncture stimulation, spinal cord injury, glial fibrillary acidic protein, Du Meridian, Stomach Meridian, nerve regeneration

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