中国组织工程研究

中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (18): 2888-2893.doi: 10.12307/2022.699

• 骨科植入物 orthopedic implant • 上一篇    下一篇

壳聚糖/聚乳酸/羟基磷灰石/聚乙烯醇复合材料骨支架的制备及表征

李耀明1，姜  宏1，石永芳2   

  1. 1新疆大学机械工程学院，新疆维吾尔自治区乌鲁木齐市   830017；2新疆医科大学医学工程技术学院，新疆维吾尔自治区乌鲁木齐市   830017
  • 收稿日期:2021-09-08 接受日期:2021-11-03 出版日期:2022-06-28 发布日期:2022-01-29
  • 通讯作者: 石永芳，副教授，新疆医科大学医学工程技术学院，新疆维吾尔自治区乌鲁木齐市 830017
  • 作者简介:李耀明，男，1996年生，河南省鹤壁市人，汉族，新疆大学在读硕士，主要从事生物3D打印方面的研究。
  • 基金资助:
    新疆维吾尔自治区自然科学基金项目(2018D01C148)，项目负责人：石永芳

Preparation and characterization of chitosan/polylactic acid/hydroxyapatite/polyvinyl alcohol composite bone scaffold

Li Yaoming1, Jiang Hong1, Shi Yongfang2   

  1. 1School of Mechanical Engineering, Xinjiang University, Urumqi 830017, Xinjiang Uygur Autonomous Region, China; 2School of Medical Engineering and Technology, Xinjiang Medical University, Urumqi 830017, Xinjiang Uygur Autonomous Region, China
  • Received:2021-09-08 Accepted:2021-11-03 Online:2022-06-28 Published:2022-01-29
  • Contact: Shi Yongfang, Associate professor, School of Medical Engineering and Technology, Xinjiang Medical University, Urumqi 830017, Xinjiang Uygur Autonomous Region, China
  • About author:Li Yaoming, Master candidate, School of Mechanical Engineering, Xinjiang University, Urumqi 830017, Xinjiang Uygur Autonomous Region, China
  • Supported by:
    the National Natural Science Foundation of China, No. 2018D01C148 (to SYF)

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摘要：

文题释义：

挤出式3D打印技术：基于快速成型技术，采用机电一体化形式，由软件模块设计打印结构，配合机械运动模块与供料系统，将材料挤出并按照预设结构逐层叠加，以实现产品打印。
正交实验：用于设计多因素多水平实验，依照正交表选择具有代表性实验组，依据评价指标探究各因素对实验结果的影响程度，实现以较少的实验次数达到与全面实验等效的结果。

背景：制备可降解材料骨组织工程支架，应用于修复骨肉瘤造成的骨缺损是当前骨组织工程研究热点。
目的：确定壳聚糖/聚乳酸/羟基磷灰石/聚乙烯醇复合材料的配比，优化骨支架力学性能。
方法：基于挤出式3D打印技术制备壳聚糖/聚乳酸/羟基磷灰石/聚乙烯醇骨支架，以最大抗压强度为评价指标，设计正交实验，以乙酸浓度(1.5%，2%，2.5%，3%)、壳聚糖溶液浓度(2%，3%，4%，5%)、羟基磷灰石∶聚乳酸质量比(1∶5，1∶6，1∶7，1∶8)、羟基磷灰石/聚乳酸∶壳聚糖溶液∶聚乙烯醇凝胶质量体积比(1 g∶1 g∶0 mL，2 g∶1 g∶1 mL，2 g∶1 g∶1.2 mL，2 g∶1 g∶1.4 mL)四因素为自变量，得到最佳材料配比。表征最佳配比复合材料的力学性能、物相组成与结合方式、孔隙率与降解性能。

结果与结论：①正交实验结果显示，乙酸浓度为3%、壳聚糖溶液浓度为5%、羟基磷灰石∶聚乳酸质量比=1∶6、羟基磷灰石/聚乳酸∶壳聚糖溶液∶聚乙烯醇凝胶=2 g∶1 g∶1.4 mL时，复合材料具有良好的力学性能；②最佳配比复合材料支架具有良好的连通性，孔隙率为(52.0±1.5)%，最大抗压强度达6 MPa，X-射线衍射扫描及傅里叶红外光谱测试结果显示，复合材料的主要结合方式为物理共混；复合材料支架具有一定的降解性能，但在测试时间内失重率较低，降解性能有待进一步提高；③结果表明，壳聚糖/聚乳酸/羟基磷灰石/聚乙烯醇复合材料支架具有较高的孔隙率和良好的力学性能。

https://orcid.org/0000-0003-4016-3608 (李耀明) 

中国组织工程研究杂志出版内容重点：人工关节；骨植入物；脊柱；骨折；内固定；数字化骨科；组织工程

关键词: 正交实验, 方差分析, 挤出式3D打印, 骨组织工程支架, 力学性能, 降解性能, 失重率, 孔隙率

Abstract: BACKGROUND: Bone tissue engineering scaffolds are prepared and applied to repair bone defects caused by osteosarcoma disease, which is the current hotspot of bone tissue engineering research.  
OBJECTIVE: To determine the ratio of chitosan/polylactic acid/hydroxyapatite/polyvinyl alcohol composite material, and optimize the mechanical properties of bone scaffolds.
METHODS:  The chitosan/polylactic acid/hydroxyapatite/polyvinyl alcohol bone scaffold was prepared using extrusion-based 3D printing technology. The maximum compressive strength was used as the evaluation index to design orthogonal experiments. The acetic acid concentration (1.5%, 2%, 2.5%, 3%), chitosan solution concentration (2%, 3%, 4%, 5%), hydroxyapatite:polylactic acid mass ratio (1:5, 1:6, 1:7, 1:8), and hydroxyapatite/polylactic acid:chitosan solution:polyvinyl alcohol gel mass volume ratio (1 g: 1 g: 0 mL, 2 g: 1 g: 1 mL, 2 g: 1 g: 1.2 mL, 2 g: 1 g: 1.4 mL) were independent variables to get the best material ratio. The mechanical properties, phase composition and bonding mode, porosity and degradation properties of the optimal ratio composite material were characterized.  
RESULTS AND CONCLUSION: (1) Orthogonal experiment results exhibited that the concentration of acetic acid was 3%; the concentration of chitosan solution was 5%; the mass ratio of hydroxyapatite:polylactic acid was 1:6; the hydroxyapatite/polylactic acid:chitosan solution:polyethylene alcohol gel was 2 g:1 g:1.4 mL. The composite material had good mechanical properties. (2) The composite material scaffold with the best ratio had good connectivity; the porosity was (52.0±1.5)%; and the maximum compressive strength reached 6 MPa. X-ray diffraction scanning and Fourier infrared spectroscopy test results demonstrated that the main bonding method of composite materials was physical blending. Composite scaffolds had certain degradation properties, but the weight loss rate was low during the test time, and degradation property needs to be further improved. (3) These findings indicate that the chitosan/polylactic acid/hydroxyapatite/polyvinyl alcohol composite scaffold has high porosity and good mechanical properties.

Key words: orthogonal experiment, analysis of variance, extrusion-based 3D printing, bone tissue engineering scaffold, mechanical properties, degradation property, weight loss rate, porosity

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