中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (14): 2279-2284.doi: 10.12307/2022.495

• 组织构建综述 tissue construction review • 上一篇    下一篇

软骨细胞分化过程中SOX9的作用

杨  爽,闫景龙   

  1. 哈尔滨医科大学附属第二医院骨科,黑龙江省哈尔滨市   150086
  • 收稿日期:2021-01-26 修回日期:2021-01-28 接受日期:2021-03-27 出版日期:2022-05-18 发布日期:2021-12-22
  • 通讯作者: 闫景龙,主任医师,博士生导师,哈尔滨医科大学附属第二医院骨科,黑龙江省哈尔滨市 150086
  • 作者简介:杨爽,男,1994年生,湖北省孝感市人,汉族,哈尔滨医科大学在读硕士,主要从事骨组织工程的研究。

Effects of SOX9 on chondrocyte differentiation

Yang Shuang, Yan Jinglong   

  1. Department of Orthopedics, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
  • Received:2021-01-26 Revised:2021-01-28 Accepted:2021-03-27 Online:2022-05-18 Published:2021-12-22
  • Contact: Yan Jinglong, Chief physician, Doctoral supervisor, Department of Orthopedics, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
  • About author:Yang Shuang, Master candidate, Department of Orthopedics, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China

摘要:

文题释义:
SOX9:SRY(性别决定区域Y)相关的高迁移率族框9(SOX9)是发育和成人软骨中的关键转录因子。它的基因从多能骨骼祖细胞表达,并且在整个软骨细胞分化过程中都具有活性。SOX9是软骨形成所必需的:它确保软骨细胞谱系的定型,是促进细胞存活并转录激活许多软骨特异性结构成分和调节因子的基因。
转录因子:是一群能与基因5`端上游特定序列专一性结合,从而保证目的基因以特定的强度在特定的时间与空间表达的蛋白质分子。

背景:软骨细胞是软骨修复工程种子细胞的来源,软骨细胞分化与软骨内骨化密切相关,软骨内骨化是必不可少的骨骼发育过程。SOX9对软骨细胞调控起着重要作用。
目的:综述SOX9在不同水平对软骨细胞分化的调控机制的最新研究进展。
方法:应用计算机检索 CNKI、PubMed及万方医学数据库从建库至 2020年12月发表的相关文献,中文关键词为“软骨,软骨细胞,SOX9基因表达调控,转录后调控,蛋白质加工,翻译后修饰,功能伙伴”;英文关键词为“Cartilage,chondrocyte,posttranscriptional regulation, posttranslational modification,SOX9,transcriptional regulation,Gene Expression Regulation,SOX9 Transcription Factor”。最终纳入61 篇符合标准的文献进行综述。
结果与结论:在基因、RNA和蛋白质水平上参与SOX9调控的关键机制已经被发现,通过SOX9关键因子作用可以直接或间接促进软骨细胞成骨生长,有正向促进,也有反向抑制,以及各因素之间相互作用。SOX9是软骨细胞分化及促进软骨形成的关键因子;通过系统分析SOX9在不同水平调控的分子机制为软骨组织工程化提供理论基础,可以更快实现软骨疾病的临床治疗应用。

https://orcid.org/0000-0003-3866-3400 (杨爽) 

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程

关键词: 软骨, 软骨细胞, SOX9, 基因表达调控, 转录, 细胞分化, mRNA, 骨关节炎

Abstract: BACKGROUND: Chondrocytes are the source of seed cells for cartilage repair engineering. Chondrocyte differentiation is closely related to endochondral ossification, which is an essential for bone development. SOX9 plays an important role in the regulation of chondrocytes.
OBJECTIVE: To review the latest research progress in the regulatory mechanism of SOX9 on chondrocyte differentiation at different levels.
METHODS: A computer search was conducted for the relevant literatures published in CNKI, WanFang, and PubMed until December 2020. The key words were “cartilage, chondrocyte, regulation of SOX9 gene expression, posttranscriptional regulation, protein processing, posttranslational modification, functional partner” in Chinese and “cartilage, chondrocyte, posttranscriptional regulation, posttranslational modification, SOX9, transcriptional regulation, gene expression regulation, SOX9 transcription factor“ in English, respectively. Finally, 61 eligible articles were included for further review.
RESULTS AND CONCLUSION: The key mechanisms involved in SOX9 regulation at the gene, RNA, and protein levels have been discovered. The SOX9 key factors can directly or indirectly promote osteogenic growth of chondrocytes, either positively or negatively, or even due to the interaction between various factors. SOX9 is a key factor in chondrocyte differentiation and chondrogenesis. By systematically analyzing the molecular mechanism of SOX9 regulation at different levels, the theoretical basis for cartilage tissue engineering can be provided, to accelerate the clinical treatment and application in cartilage diseases.

Key words: cartilage, chondrocytes, SOX9, gene expression regulation, transcription, cell differentiation, mRNA, osteoarthritis

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