Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (31): 5722-5725.doi: 10.3969/j.issn.1673-8225.2011.31.005

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Effects of Danshen on heme oxygenase-1 expression in rat transplanted liver

Gao Chuan-chang, Li Dong-sheng, Wang Kai, Zou Shu-bing   

  1. Department of Hepatobiliary Surgery, Second Affiliated Hospital of Medical College of Nanchang University, Nanchang 330006, Jiangxi Province, China
  • Received:2011-04-07 Revised:2011-06-10 Online:2011-07-30 Published:2011-07-30
  • Contact: Zou Shu-bing, Professor, Chief physician, Master’s supervisor, Department of Hepatobiliary Surgery, Second Affiliated Hospital of Medical College of Nanchang University, Nanchang 330006, Jiangxi Province, China zousb999@163.com
  • About author:Gao Chuan-chang★, Studing for master’s degree, Department of Hepatobiliary Surgery, Second Affiliated Hospital of Medical College of Nanchang University, Nanchang 330006, Jiangxi Province, China chuanzhang1985116@yahoo.cn

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Abstract:

BACKGROUND: Danshen pretreatment before organ transplantation can protect tissue from ischemia-reperfusion injury and improve the survival rate of grafts
OBJECTIVE: To investigate the effects of Danshen-containing cold perfusion solution on heme oxygenase-1 (HO-1) expression in rat liver allograft and the protective effects on donor liver from ischemia-reperfusion injury.
METHODS: Sprague-Dawley male rats were randomly divided into University of Wisconsin solution (UW) solution group (UW group, preservation using UW solution during transplantation), Danshen+UW solution group (DS+UW group, preservation with Danshen+UW solution during transplantation), ZnPP (HO-1 inhibitor) pretreatment group (ZnPP group, intraperitoneal injection of ZnPP 24 hours before transplantation, and preservation with Danshen+UW solution during transplantation). Reliable rat models of liver allografting were established. Ten normal rats were selected as normal controls.
RESULTS AND CONCLUSION: Serum total bilirubin, alanine aminotransferase, and aspartate aminotransferase levels were significantly lower in the DS+UW group, UW group than in the ZnPP group (P < 0.01). HO-1 mRNA and protein expressions were greater in the DS+UW group than in the UW group, but the expressions were obviously inhibited in the ZnPP group (P < 0.05). Suzuki scores were significantly lower in the DS+UW group than in the ZnPP group and UW group. These findings suggest that Danshen can upregulate HO-1 mRNA and protein expressions in rat liver allograft, alleviate ischemia-reperfusion injury of donor liver, and protect the transplanted liver.

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