Erythropoietin effects on expression of stem cell factor and its receptor in acute renal injury

doi:10.3969/j.issn.2095-4344.2013.53.017

Abstract

Abstract:

BACKGROUND: The effects of cytokines on lessening ischemia/reperfusion injury to the kidney have been paid attention increasingly. Stem cell factor has protective effects on organs except hematopoietic system.

OBJECTIVE: To study the expression of stem cell factor and its receptor c-Kit in acute kidney injury model of rat, and the effect of erythropoietin on the expression of stem cell factor and c-Kit.

METHODS: A total of 34 adult male Wistar rats were selected to induce ischemia/reperfusion models by occluding bilateral renal pedicle (ischemia for 45 minutes and reperfusion for 24 hours). The animals were randomly divided into sham-operation group (n=10), ischemia/reperfusion group (n=12) and erythropoietin group (n=12). In the erythropoietin group, 5 000 U/kg recombinant human erythropoietin was injected once via the tail vein at 2 hours before modeling. The expression of stem cell factor and c-Kit was detected by immunohistochemistry and image analysis technique in each group. The serum concentrations of creatinine and blood urea nitrogen were measured. Meanwhile, renal pathologic changes were observed by hematoxylin-eosin staining, and renal tubule injury integral was calculated.

RESULTS AND CONCLUSION: Stem cell factor and c-Kit were detected only in the renal tubule section. The expression of stem cell factor and c-Kit in the ischemia/reperfusion group and erythropoietin group was higher than sham-operation group (P < 0.05). The expression of stem cell factor was higher in the erythropoietin group than that in the ischemia/reperfusion group (P < 0.01). However, no significant difference in c-Kit was detected between the two groups (P > 0.05). Serum creatinine and urea nitrogen levels were significantly lower in the erythropoietin group than that in the ischemia/reperfusion group (P < 0.05), but higher than that in the sham-operation group (P < 0.05). Compared with the ischemia/reperfusion group, pathological changes in nephridial tissue were more lessened in the erythropoietin group. These results suggested that stem cell factor and c-Kit expression increased when ischemia/reperfusion led to acute renal injury. The protective effect of erythropoietin on acute renal injury possibly associated with the upregulation of stem cell factor and c-Kit expression.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: stem cells, stem cell factor, kidney, reperfusion injury, erythropoietin

CLC Number:

R318

Wang Qing-xia, Li Hong-jie, Liu Xue-mei, Xu Yan. Erythropoietin effects on expression of stem cell factor and its receptor in acute renal injury[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(53): 9203-9208.

Figures/Tables 4

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