Histocompatibility between small intestinal submucosa and synovial mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2013.47.010

Abstract

Abstract:

BACKGROUND: Small intestinal submucosa is characterized as antimicrobial activity, good biocompatibility, bio-mechanical properties, and rapid degradation in vivo, similar to the extracellular matrix of meniscal fibrochondrocytes.
OBJECTIVE: To observe whether there exists a good histocompatibility between small intestinal submucosa and synovial mesenchymal stem cells.
METHODS: Small intestinal submucosa was treated with physical and chemical treatment. And hematoxylin-eosin staining and scanning electron microscopy observation were performed. Then, small intestinal submucosa extracts were prepared for the following experiments. (1) Pyrogenic test: small intestinal submucosa extracts were injected into the ear vein of New Zealand white rabbits. (2) Skin sensitization test: small intestinal submucosa extracts, paraformaldehyde solution and normal saline were respectively injected intradermally into New Zealand white rabbits. (3) General toxicity test: small intestinal submucosa extracts and normal saline were respectively injected into the ear vein of New Zealand white rabbits. Small intestinal submucosa was co-cultured with osteogenic rabbit synovial mesenchymal stem cells, and small intestinal submucosa cultured alone served as control.
RESULTS AND CONCLUSION: There were some intestinal mucosal epithelial cells, fat cells and other cells adhered onto the surface of small intestinal submucosa after physical treatment. While, the amount of residual cells decreased sharply after chemical treatment. But the main structure and the component had not been changed. The surface of small intestinal submucosa was smooth and no cells remained, and there was a three-dimension network spatial structure. The porosity was 80%. Small intestinal submucosa is a non-toxic, nonirritating, non-immunogenic biomaterial with very good biocompatibility, which has a good histocompatibility with rabbit synovial mesenchymal stem cells.

Key words: biocompatible materials, histocompatibility, bone matrix, extracellular matrix

CLC Number:

R318

Fu Pei-liang, Zhang Lei, Wu Yu-li, Wu Hai-shan, Cong Rui-jun, Chen Song, Ding Zhe-ru, Zhou Qi. Histocompatibility between small intestinal submucosa and synovial mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(47): 8202-8208.

Figures/Tables 6

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