Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (18): 3306-3310.doi: 10.3969/j.issn.1673-8225.2011.18.019

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Contribution of mouse CD1d to immune toleration induced by immature dendritic cells in vitro

Ma Zhao-hui1, Han Yong2, Yao Jia-lin3, Yao Zhi-fa3   

  1. 1Department of Cardiac Surgery, the 211 Hospital of Chinese PLA, Harbin  150080, Heilongjiang Province, China
    2Tangdu Hospital, Fourth Military Medical University of Chinese PLA, Xi’an  710038, Shaanxi Province, China
    3Second Affiliated Hospital, Harbin Medical University, Harbin  150080, Heilongjiang Province, China
  • Received:2010-12-13 Revised:2011-03-26 Online:2011-04-30 Published:2011-04-30
  • About author:Ma Zhao-hui☆, Doctor, Chief physician, Department of Cardiac Surgery, the 211 Hospital of Chinese PLA, Harbin 150080, Heilongjiang Province, China mazhaohui211@163.com

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Abstract:

BACKGROUND: Dendritic cells (DCs) possess two-way immune regulation ability: mature DCs (mDCs) can activate the immune response, and immature DCs (imDCs) tend to induce immune tolerance.
OBJECTIVE: To discuss the role of CD1d in immune toleration induced by imDCs after transplantation and the mechanism of cytokine played in this process.
METHODS: imDCs of BALB/c mouse transfected by viral interleukin-10 were stimulated with GM-CSF, LPS in vitro, and separated into three groups after anti-CD1d intervention. The expression of cytokine and phenotypes of the cells were observed. 1st MLC and 2nd MLC were performed in different experimental conditions to observe the immunity of CD1d with heterogeneity T lymphocyte existing. Proliferation of T lymphocyte and the expression of cytokine were observed.
RESULTS AND CONCLUSION: Proliferation of heterogeneity T lymphocyte induced by imDC was suppressed by anti-CD1d intervention. The immune function of heterogeneity T lymphocyte was deficient after sensitized by imDCs with anti-CD1d intervention. Anti-CD1d intervention would affect the functional maturity of imDCs in case the heterogeneity T lymphocyte existed, in that it could suppress the secretion of Th1 type cytokine interleukin-12, however enhance the secretion of Th2 type cytokine interleukin-10.

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