Intranasal administration of the conditioned medium of human umbilical cord-derived mesenchymal stem cells for treatment of cerebral ischemia-reperfusion injury

doi:10.3969/j.issn.2095-4344.2013.45.011

Abstract

Abstract:

BACKGROUND: Cytokines and neurotrophic factors secreted from human umbilical cord blood-derived mesenchymal stem cells secrete have neuroprotective effects on cerebral ischemia-reperfusion injury, but there are few reports about intranasal administration of human umbilical cord blood-derived mesenchymal stem cell conditioned medium in the treatment of stroke.
OBJECTIVE: To investigate the protective effects of intranasal administration of human umbilical cord-derived mesenchymal stem cells-conditioned medium on neurologic function of rats with cerebral ischemia-reperfusion injury.
METHODS: Adult rats were subjected to 2 hours of right middle cerebral artery occlusion and the human umbilical cord-derived mesenchymal stem cells were isolated from the postpartum human cord. We made the conditioned medium of human umbilical cord-derived mesenchymal stem cells. Ischemic rats were randomized and assigned to three groups and were treated by intranasal routine starting 24 hours after middle cerebral artery occlusion with: (1) saline for control group; (2) Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12 medium for medium control group; (3) conditioned medium treatment group (10mL/kg) daily for 14 days. Behavioral tests (foot fault test, and modified Neurological Severity Score) were performed before and at 1, 7, 14 days after middle cerebral artery occlusion.
RESULTS AND CONCLUSION: There was no difference in the behavioral tests among the three groups at postoperatively 1 day (P > 0.05). Compared to the control and medium control group rats, respectively, rats in the conditioned medium group significantly improved functional outcome after stroke in days 7 and 14 (P < 0.05). There was also no significant difference in functional tests between the control group and medium control group in days 7 and 14 (P > 0.05). These results suggest that human umbilical cord-derived mesenchymal stem cells-conditioned medium via intranasal administration can significantly improve neurologic functional outcome after cerebral ischemia-reperfusion injury.

Key words: stem cells, fetal blood, mesenchymal stem cells, culture media, conditioned, administration, intranasal, reperfusion injury, infarction, middle cerebral artery

CLC Number:

R394.2

Shen Li-ping, Wang Shuai-shuai, Dong Li-guo, Shen Xia, Hua Fang, Ye Xin-chun, Cui Gui-yun. Intranasal administration of the conditioned medium of human umbilical cord-derived mesenchymal stem cells for treatment of cerebral ischemia-reperfusion injury[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.45.011.

Figures/Tables 2

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