中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (41): 7612-7616.doi: 10.3969/j.issn.2095-4344.2012.41.003

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

刘永琦1，2，达 瑞1，窦娟娟1，苏菊萍1，颜春鲁1   

  1. 1甘肃中医学院，甘肃省兰州市 730030
    2甘肃省中药药理与毒理学省级重点实验室中西医结合基础室，甘肃省兰州市 730030
  • 收稿日期:2012-01-07 修回日期:2012-02-20 出版日期:2012-10-07 发布日期:2012-10-07
  • 作者简介:刘永琦★，男，1973年生，汉族，2002年成都中医药大学毕业，硕士，教授，硕士生导师，主要从事中医药免疫学及干细胞生物学与中医药科学研究。 liuyongqi73@163.com

Effects of cadmium chloride on micronucleus rate and chromosome aberration rate in mouse bone marrow mesenchymal stem cells

Liu Yong-qi1,2, Da Rui1, Dou Juan-juan1, Su Ju-ping1, Yan Chun-lu1   

  1. 1Gansu University of Traditional Chinese Medicine, Lanzhou 730000, Gansu Province, China
    2Room of Integrated Chinese and Western Medicine, Gansu Province Laboratory of Pharmacology and Toxicology of Traditional Chinese Medicine, Lanzhou 73000, Gansu Province, China
  • Received:2012-01-07 Revised:2012-02-20 Online:2012-10-07 Published:2012-10-07
  • About author:Liu Yong-qi★, Master, Professor, Master’s supervisor, Gansu University of Traditional Chinese Medicine, Lanzhou 730000, Gansu Province, China liuyongqi73@163.com

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摘要：

背景：氯化镉对骨髓间充质干细胞遗传方面影响的研究暂无报道。
目的：观察化学毒性物质氯化镉对骨髓间充质干细胞微核率和染色体畸变率的影响。
方法：贴壁培养小鼠骨髓间充质干细胞细胞株，显微镜下观察空白对照组及氯化镉诱导组骨髓间充质干细胞的形态学变化，微核实验检测两组微核率，染色体分析检测两组染色体畸变率。
结果与结论：显微镜下观察，空白对照组细胞呈成纤维细胞样贴壁生长，胞体透亮，折光性强；氯化镉诱导组细胞呈凸起回缩，细胞变小、变圆，贴壁不牢，悬浮细胞增多。与空白对照组比较，氯化镉诱导组骨髓间充质干细胞微核发生率和染色体畸变率均显著增加(P < 0.05)。提示氯化镉可引起小鼠骨髓间充质干细胞遗传物质的损伤。

关键词: 骨髓间充质干细胞, 氯化镉, 微核率, 染色体畸变率, 干细胞

Abstract:

BACKGROUND: There have been no reports describing the effects of cadmium chloride on heredity of bone marrow mesenchymal stem cells (BMSCs)
OBJECTIVE: To investigate the effects of cadmium chloride on micronucleus rate and chromosome aberration rate in mouse BMSCs.
METHODS: Mouse BMSCs were cultured by adherence method. Morphology of BMSCs was observed in the blank control group and cadmium chloride-induced group. The micronucleus rate and chromosome aberration rate in mouse BMSCs were determined.
RESULTS AND CONCLUSION: Under the microscope, cells in the blank control group grew adherently like fibroblasts, with transparent soma and strong refraction; in the cadmium chloride-induced group, cells were retracted, somas became small and round, and were weakly adherent, and suspension cells increased. The micronucleus rate and chromosome aberration rate in mouse BMSCs were significantly increased in the cadmium chloride-induced group than in the blank control group (P < 0.05). These findings suggest that cadmium chloride can cause damage to BMSC genetic material.

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